中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2015年
7期
515-518
,共4页
黄川锋%王海鑫%康爱英%张国良%焦志峰%丁可
黃川鋒%王海鑫%康愛英%張國良%焦誌峰%丁可
황천봉%왕해흠%강애영%장국량%초지봉%정가
辛夷%酒精性肝损伤%氧化应激%细胞色素P4502 E1
辛夷%酒精性肝損傷%氧化應激%細胞色素P4502 E1
신이%주정성간손상%양화응격%세포색소P4502 E1
Magnolia biondii Pamp%alcoholic liver injury%oxidative stress%cytochrome P450 2 E1 mRNA
目的：研究辛夷对急性酒精性肝损伤小鼠肝抗氧化功能和组织病理改变的影响及可能机制。方法将50只雄性昆明种小鼠随机分为5组：空白组、模型组和低、中、高剂量辛夷组（1.0，2.0，4.0 g· kg－1），各组连续灌胃10 d。用白酒（12 mL· kg－1）灌胃法建立急性酒精性肝损伤小鼠模型。测定血清谷丙转氨酶（ALT）、谷草转氨酶（AST）活性；测定肝组织丙二醛（MDA）含量和谷胱甘肽过氧化物酶（GSH－PX）活性，实时荧光定量PCR检测肝组织细胞色素氧化酶P4502E1（CYP2E1）mRNA表达水平。肝组织HE染色观察病理改变。结果和模型组比较，辛夷中、高剂量组小鼠血清ALT、AST活性和肝组织MDA含量、CYP2E1 mRNA表达水平显著降低，而血清GSH－PX含量明显增高（均P＜0.05），肝细胞变性、坏死、炎性细胞浸润程度明显减轻。结论辛夷对小鼠酒精性肝损伤具有明显的保护作用，可能与降低CYP2E1 mRNA表达水平和氧化应激作用有关。
目的：研究辛夷對急性酒精性肝損傷小鼠肝抗氧化功能和組織病理改變的影響及可能機製。方法將50隻雄性昆明種小鼠隨機分為5組：空白組、模型組和低、中、高劑量辛夷組（1.0，2.0，4.0 g· kg－1），各組連續灌胃10 d。用白酒（12 mL· kg－1）灌胃法建立急性酒精性肝損傷小鼠模型。測定血清穀丙轉氨酶（ALT）、穀草轉氨酶（AST）活性；測定肝組織丙二醛（MDA）含量和穀胱甘肽過氧化物酶（GSH－PX）活性，實時熒光定量PCR檢測肝組織細胞色素氧化酶P4502E1（CYP2E1）mRNA錶達水平。肝組織HE染色觀察病理改變。結果和模型組比較，辛夷中、高劑量組小鼠血清ALT、AST活性和肝組織MDA含量、CYP2E1 mRNA錶達水平顯著降低，而血清GSH－PX含量明顯增高（均P＜0.05），肝細胞變性、壞死、炎性細胞浸潤程度明顯減輕。結論辛夷對小鼠酒精性肝損傷具有明顯的保護作用，可能與降低CYP2E1 mRNA錶達水平和氧化應激作用有關。
목적：연구신이대급성주정성간손상소서간항양화공능화조직병리개변적영향급가능궤제。방법장50지웅성곤명충소서수궤분위5조：공백조、모형조화저、중、고제량신이조（1.0，2.0，4.0 g· kg－1），각조련속관위10 d。용백주（12 mL· kg－1）관위법건립급성주정성간손상소서모형。측정혈청곡병전안매（ALT）、곡초전안매（AST）활성；측정간조직병이철（MDA）함량화곡광감태과양화물매（GSH－PX）활성，실시형광정량PCR검측간조직세포색소양화매P4502E1（CYP2E1）mRNA표체수평。간조직HE염색관찰병리개변。결과화모형조비교，신이중、고제량조소서혈청ALT、AST활성화간조직MDA함량、CYP2E1 mRNA표체수평현저강저，이혈청GSH－PX함량명현증고（균P＜0.05），간세포변성、배사、염성세포침윤정도명현감경。결론신이대소서주정성간손상구유명현적보호작용，가능여강저CYP2E1 mRNA표체수평화양화응격작용유관。
Objective To study the effect of Magnolia biondii Pamp ( MBP ) on hepatic antioxidant function and pathological histology for alcoholic liver injury in mice.Methods Fifty male KM mice were ran-domly divided into five groups as below:blank group , acute alcohol liver injury model group , MBP low -, medium -and high -dose groups (1.0, 2.0, 4.0 g· kg -1 ).The acute alcohol liver injury mouse model was set up by intragastric administration of alcohol once daily for 10 days.The activity of serum alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , glutathione peroxidase ( GSH -PX ) activity and malondialdehyde ( MDA) content in liver were measured.The ex-pression of cytochrome P450 2E1 (CYP2E1) mRNA from the liver were determined by real-time fluorescent quantitative PCR.The pathological changes of liver were observed by light microscope with HE staining.Results Compared with the model group , MBP medium -and high-dose groups have little pathological damage of liver.The levels of ALT, AST, MDA and CYP2E1 mRNA were significant decreased while GSH-PX was significant increased.Conclusion MBP have a signifi-cant protective effect on acute alcohol liver injury in mice , which exerted its beneficial effects mainly through inhibiting oxidative damage and de-creased the expression of CYP 2E1 mRNA.
