CAJ | 학술논문

目的：研究肺腺癌转移相关转录子1（ MALAT1）对人宫颈癌癌Hela细胞顺铂化疗敏感性的影响。方法用RNA干扰（ RNAi）技术下调MALAT1在人宫颈癌Hela细胞中的表达，对Hela细胞用顺铂1 mg· L－1进行处理。用细胞计数法检测不同时间点Hela细胞增殖能力的变化，用流式细胞检测Hela细胞周期变化。结果 RNAi下调MALAT1表达后，Hela细胞对顺铂化疗敏感性显著增高，而细胞增殖能力明显下降（均 P ＜0.05）。 G0／G1期细胞比率明显增高（ P＜0.05），Hela细胞迁移能力显著降低（ P＜0.05）。结论抑制长链非编码RNA MALAT1可增强人宫颈癌Hela细胞对顺铂化疗的敏感性。
목적：연구폐선암전이상관전록자1（ MALAT1）대인궁경암암Hela세포순박화료민감성적영향。방법용RNA간우（ RNAi）기술하조MALAT1재인궁경암Hela세포중적표체，대Hela세포용순박1 mg· L－1진행처리。용세포계수법검측불동시간점Hela세포증식능력적변화，용류식세포검측Hela세포주기변화。결과 RNAi하조MALAT1표체후，Hela세포대순박화료민감성현저증고，이세포증식능력명현하강（균 P ＜0.05）。 G0／G1기세포비솔명현증고（ P＜0.05），Hela세포천이능력현저강저（ P＜0.05）。결론억제장련비편마RNA MALAT1가증강인궁경암Hela세포대순박화료적민감성。
Objective To evaluate the biological function of metastasis-associated lung adenocarcinoma transcript 1 ( MALAT1 ) associated with cisplatin resistance in Hela cell.Methods MALAT1 expression was know down by RNA interfere technology in human cervical carcinoma cell Hela.And the Hela cell was treated by 10 mg · L-1 cisplatin.The cell proliferation was tested by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 -H -tetrazolium bromide ( MTT ) array and cell cycle was tested by Flow cytometry assay.Results The cell proliferation was significant inhibit when decrease the expression in Hela cell ( P<0.05 ) . Which demonstrated that the inhibit MALAT 1 Hela cell was sensitive to cisplatin.And the G0/G1 cell cycle proportion was significant increased than the control group ( P <0.05 ).The migration ability in the si-MALAT1 Hela cell was significant decreased ( P<0.05 ).Conclu-sion Know down MALAT1expression can significant increase the cispla-tin sensitivity in human cervical cancer Hela cell.