CAJ | 학술논문

目的：研究人胆管癌细胞在塞来昔布和放疗作用下发生凋亡的作用机制。方法将QBC939细胞株分为四组院对照组、塞来昔布组（C组）、放疗组（R组）和塞来昔布联合放疗组（C+R组，联合组），分别进行不同浓度的塞来昔布和不同剂量X线作用后，采用CCK-8法、流式细胞技术和Western blot等方法分别检测各组细胞生长、凋亡率及survivin蛋白的表达。结果联合组的凋亡率明显增加，从3.527%升至23.364%（P<0.05），survivin蛋白表达明显降低（P<0.05）。结论应用塞来昔布对接受放疗的人QBC939细胞株有凋亡增敏作用，下调survivin的表达是其机制之一。
목적：연구인담관암세포재새래석포화방료작용하발생조망적작용궤제。방법장QBC939세포주분위사조원대조조、새래석포조（C조）、방료조（R조）화새래석포연합방료조（C+R조，연합조），분별진행불동농도적새래석포화불동제량X선작용후，채용CCK-8법、류식세포기술화Western blot등방법분별검측각조세포생장、조망솔급survivin단백적표체。결과연합조적조망솔명현증가，종3.527%승지23.364%（P<0.05），survivin단백표체명현강저（P<0.05）。결론응용새래석포대접수방료적인QBC939세포주유조망증민작용，하조survivin적표체시기궤제지일。
Objective To investigate the effect of celecoxib in combination with radiotherapy on the apoptosis of human cholangiocarcinoma cell line QBC939 in vitro. Methods The QBC939 cells were maintained in RIMP1640 supple-mented with 10% FBS and treated with celecoxib in combination with x-ray. Cell Counting Kit-8 (CCK-8)assay was used to investigate the inhibition ratio and the IC50. Cells were divided into four groups including control,radiotherapy,celecoxib, radiotherapy and celecoxib. Apoptosis of each group was determined by flow cytometry. Expression of sur-vivin was determined by western blot. Results The apoptosis ratio of the combination treatment groups were enhanced apparently,from 3.527%of control group to 23.364%(P<0.05). Survivin expression was down-regulated obviously after combination treatment in western blot. Conclusion Celecoxib could enhance radiotherapy's effect of apoptosis-induc-ing. Celecoxib has the radiosensitivity-enchancing effect on the cholangiocarcinoma QBC939 cells in vitro,down regu-lation of survivin expression may be one of the possible mechanisms.