CAJ | 학술논문

目的：探讨七氟醚对新生大鼠海马 N －甲基－D －天冬氨酸受体（ NMDA）亚基mRNA表达的影响。方法新生SD大鼠随机分为2组（均12只），七氟醚组吸入3％七氟醚6 h，而对照组吸入30％氧气6 h。 2组大鼠吸入气体后24 h处死，取海马组织，用原位杂交法检测海马NMDA受体亚基mRNA表达及 NMDA 受体2B 亚基（ NR2B）与 NMDA 受体2A 亚基（NR2A）比率的变化。结果与对照组比较，七氟醚组NR2AmRNA表达显著下调（P＜0.05），NR2BmRNA表达显著上调（P＜0.05），NR2B／NR2A的比率显著上升（ P＜0.05）。结论七氟醚诱发新生鼠海马神经毒性可能与调控NMDA受体亚基mRNA表达有关。
목적：탐토칠불미대신생대서해마 N －갑기－D －천동안산수체（ NMDA）아기mRNA표체적영향。방법신생SD대서수궤분위2조（균12지），칠불미조흡입3％칠불미6 h，이대조조흡입30％양기6 h。 2조대서흡입기체후24 h처사，취해마조직，용원위잡교법검측해마NMDA수체아기mRNA표체급 NMDA 수체2B 아기（ NR2B）여 NMDA 수체2A 아기（NR2A）비솔적변화。결과여대조조비교，칠불미조NR2AmRNA표체현저하조（P＜0.05），NR2BmRNA표체현저상조（P＜0.05），NR2B／NR2A적비솔현저상승（ P＜0.05）。결론칠불미유발신생서해마신경독성가능여조공NMDA수체아기mRNA표체유관。
Objective To investigate the effect of sevoflurane on mRNA expression of N -methyl -D -aspartate ( NMDA ) receptor subunit in neonatal rat hippocampi.Methods Twenty-four SD rats were devided into 2 groups( n=12 each) via a random number table:sevoflurane group and control group.The 3.0% sevoflurane and oxy-gen inhalated for 6 h respectively.Rats were sacrificed at 24 h after inhalation of sevoflurane or oxygen.Hippocampi were removed to idendtify the mRNA expression of NMDA subunit and the ratio of NR2 B/NR2 A through in situ hybridization.Results Compared with control group, NR2BmRNA and the ratio of NR2B/NR2A increased, and NR2 AmRNA decreased significantly in sevoflurane group ( P<0.05 ).Conclusion Neurotoxicity of sevoflurane in immature hippocampi might be attributed to regulation of mRNA expression of NMDA subunit in neonatal rat.