CAJ | 학술논문

目的：探讨对吉西他滨( GEM )耐药的胰腺癌SW1990细胞诱导方法,以期进一步认识胰腺癌耐药的发生机制,并对诱导的GEM耐药的胰腺癌细胞与其亲本细胞的生物学特性进行比较。方法采用逐步浓度递增法诱导对GEM耐药的细胞株 SW1990-GZ。 MTT 法检测 SW1990和SW1990-GZ 的半数致死量(IC50)、耐药系数(R),并观察其生长差异。采用高效液相色谱法( HPLC)检测不同时间点SW1990和 SW1990-GZ 对 GEM 药物的摄取情况。结果SW1990和SW1990-GZ的IC50为(0．07±0．0021)、(87．50±3．2400)μg/ml,R=1250；在不同浓度GEM作用下,诱导后耐药的SW1990-GZ对GEM的敏感性明显降低。细胞生长曲线显示耐药细胞 SW1990-GZ相对于 SW1990生长缓慢。不同时间点GEM孵育后,SW1990-GZ细胞对GEM药物的摄取较SW1990细胞降低。结论成功诱导了耐GEM药物的SW1990-GZ细胞株,耐药性能稳定、明显。 SW1990-GZ细胞可能存在着一定的机制使得细胞摄取GEM降低。
목적：탐토대길서타빈( GEM )내약적이선암SW1990세포유도방법,이기진일보인식이선암내약적발생궤제,병대유도적GEM내약적이선암세포여기친본세포적생물학특성진행비교。방법채용축보농도체증법유도대GEM내약적세포주 SW1990-GZ。 MTT 법검측 SW1990화SW1990-GZ 적반수치사량(IC50)、내약계수(R),병관찰기생장차이。채용고효액상색보법( HPLC)검측불동시간점SW1990화 SW1990-GZ 대 GEM 약물적섭취정황。결과SW1990화SW1990-GZ적IC50위(0．07±0．0021)、(87．50±3．2400)μg/ml,R=1250；재불동농도GEM작용하,유도후내약적SW1990-GZ대GEM적민감성명현강저。세포생장곡선현시내약세포 SW1990-GZ상대우 SW1990생장완만。불동시간점GEM부육후,SW1990-GZ세포대GEM약물적섭취교SW1990세포강저。결론성공유도료내GEM약물적SW1990-GZ세포주,내약성능은정、명현。 SW1990-GZ세포가능존재착일정적궤제사득세포섭취GEM강저。
Objective To investigate how to induce the resistant cell line SW1990/gemcitabine( GEM) for further clarify the resistant mechanisms of pancreatic cancer , and compare the characteristics between SW1990 and SW1990-GZ. Methods SW1990-GZ was derived from the human pancreatic cancer cell lines SW1990 by exposing the cells to intermittently increasing concentrations of GEM. The IC50 ,resistance index( R) , and growth difference was observed by MTT assay. Cellular intake of SW1990 and SW1990-GZ was detection by HPLC. Results The IC50 of SW1990 and SW1990-GZ were respectively (0. 07 ± 0. 002 1)μg/ml,(87. 50 ± 3. 240 0)μg/ml,R=1 250;sensibility to GEM in SW1990-GZ were more lower than that in SW1990 cell in different concentration s of GEM. Growth rate of SW1990-GZ was slower than that of SW1990 from the growth curve;GEM intake in SW1990-GZ was lower than that in SW1990 in different times. Conclusion Human resistant pancreatic cancer cell lines SW1990/GEM is successfully established,and its resistant characteristics are stable and clear. SW1990-GZ maybe decrease GEM intake by some unclear pathways.