安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
4期
437-440
,共4页
刘祥%张金颖%张永萍%仲英娜
劉祥%張金穎%張永萍%仲英娜
류상%장금영%장영평%중영나
白介素-28B%丙型病毒性肝炎%基因多态性%易感性
白介素-28B%丙型病毒性肝炎%基因多態性%易感性
백개소-28B%병형병독성간염%기인다태성%역감성
interleukin-28 B%hepatitis C%gene polymorphisms%susceptibility
目的:探讨白介素-28B( IL-28B)基因的多态性与新疆维吾尔族慢性丙型肝炎感染易感性的关联。方法选取180例新疆维吾尔族慢性丙肝患者作为HCV组,150例维族健康体检者作为健康对照组,通过PCR产物测序法检测两组受试者IL-28B rs8099917、rs12979860基因型和等位基因分布频率,对两组基因型和等位基因差异进行分析;同时在HCV组中,对不同IL-28B基因型人群的HCV-RNA载量、肝功能、血脂等进行分析。结果①rs8099917在HCV组和健康对照组中TT和TG/GG基因型频率差异有统计学意义(χ2=11.627,P=0.001,OR=1.363,95%CI:1.107~1.591);G等位基因率在两组间差异明显,HCV组中占10.6%,健康对照组0.17%(χ2=7.011, P =0.008, OR =0.658,95%CI:0.512~0.905);② rs12979860在两组间的基因型和等位基因频率差异无统计学意义;③rs12979860 TT型患者的HCV-RNA高于TG/GG型,差异有统计学意义( P=0.032);患者肝功血脂差异与IL-28B基因多态性中无关。结论新疆维吾尔族人群中HCV的易感性与IL-28B rs8099917基因多态性有关联,rs8099917中等位基因G是新疆维族HCV患者的易感基因。
目的:探討白介素-28B( IL-28B)基因的多態性與新疆維吾爾族慢性丙型肝炎感染易感性的關聯。方法選取180例新疆維吾爾族慢性丙肝患者作為HCV組,150例維族健康體檢者作為健康對照組,通過PCR產物測序法檢測兩組受試者IL-28B rs8099917、rs12979860基因型和等位基因分佈頻率,對兩組基因型和等位基因差異進行分析;同時在HCV組中,對不同IL-28B基因型人群的HCV-RNA載量、肝功能、血脂等進行分析。結果①rs8099917在HCV組和健康對照組中TT和TG/GG基因型頻率差異有統計學意義(χ2=11.627,P=0.001,OR=1.363,95%CI:1.107~1.591);G等位基因率在兩組間差異明顯,HCV組中佔10.6%,健康對照組0.17%(χ2=7.011, P =0.008, OR =0.658,95%CI:0.512~0.905);② rs12979860在兩組間的基因型和等位基因頻率差異無統計學意義;③rs12979860 TT型患者的HCV-RNA高于TG/GG型,差異有統計學意義( P=0.032);患者肝功血脂差異與IL-28B基因多態性中無關。結論新疆維吾爾族人群中HCV的易感性與IL-28B rs8099917基因多態性有關聯,rs8099917中等位基因G是新疆維族HCV患者的易感基因。
목적:탐토백개소-28B( IL-28B)기인적다태성여신강유오이족만성병형간염감염역감성적관련。방법선취180례신강유오이족만성병간환자작위HCV조,150례유족건강체검자작위건강대조조,통과PCR산물측서법검측량조수시자IL-28B rs8099917、rs12979860기인형화등위기인분포빈솔,대량조기인형화등위기인차이진행분석;동시재HCV조중,대불동IL-28B기인형인군적HCV-RNA재량、간공능、혈지등진행분석。결과①rs8099917재HCV조화건강대조조중TT화TG/GG기인형빈솔차이유통계학의의(χ2=11.627,P=0.001,OR=1.363,95%CI:1.107~1.591);G등위기인솔재량조간차이명현,HCV조중점10.6%,건강대조조0.17%(χ2=7.011, P =0.008, OR =0.658,95%CI:0.512~0.905);② rs12979860재량조간적기인형화등위기인빈솔차이무통계학의의;③rs12979860 TT형환자적HCV-RNA고우TG/GG형,차이유통계학의의( P=0.032);환자간공혈지차이여IL-28B기인다태성중무관。결론신강유오이족인군중HCV적역감성여IL-28B rs8099917기인다태성유관련,rs8099917중등위기인G시신강유족HCV환자적역감기인。
Objective To study the association between interleukin-28B (IL-28B) single nucleotide polymorphism and susceptibility to hepatitis C virus ( HCV) infection in Xinjiang Uyghur populition. Methods In this study,the IL-28 B rs8099917 and rs12979860 locus were genotyped in 180 patients infected with HCV and 150 healthy con-trols using the method of direct gene sequencing. To analyze the differences of SNPs genotypes and allele frequen-cies in two groups,SPSS 13. 0 was used. Results ① TT and TG/GG genotype frequencies of rs8099917 differed significantly between patients with chronic HCV and healthy controls (χ2 =11. 627,P=0. 001,OR=1. 363,95%CI:1. 107 ~1. 591 ) . Patients with HCV infection had a higher G allele frequency than healthy controls (χ2 =7. 011,P=0. 008,OR=0. 658,95%CI:0. 512~0. 905).② rs12979860 polymorphisms was not significantly asso-ciated with the susceptibility to HCV infection in Xinjiang Uyghur populition. Conclusion The IL-28B rs8099917 gene polymorphism correlates with susceptibility to HCV infection in Uyghur patient. G allele is associated with a higher risk of HCV infection.