CAJ | 학술논문

目的：利用生物素-亲和素系统，将生物素共价键合在钛基材表面，为钛种植体表面接枝生物活性分子提供新的实验方法。方法使用3-氨丙基膦酸( APP)对钛基材表面进行酸蚀处理并使其获得游离氨基，由碳二亚胺( EDC )介导，通过与羧基反应形成酰胺键以实现生物素-亲和素系统在钛种植体表面的固定。结果通过环境扫描电镜、傅立叶变换红外光谱、X射线光电子能谱、免疫荧光等检测方法对样品进行表征，证实钛基材表面已成功接枝生物素。结论采用生物素-亲和素系统，可以在氨基化的钛基材表面成功接枝生物素，为钛种植体表面生物化修饰的研究提供新的方法和思路。
목적：이용생물소-친화소계통，장생물소공개건합재태기재표면，위태충식체표면접지생물활성분자제공신적실험방법。방법사용3-안병기련산( APP)대태기재표면진행산식처리병사기획득유리안기，유탄이아알( EDC )개도，통과여최기반응형성선알건이실현생물소-친화소계통재태충식체표면적고정。결과통과배경소묘전경、부립협변환홍외광보、X사선광전자능보、면역형광등검측방법대양품진행표정，증실태기재표면이성공접지생물소。결론채용생물소-친화소계통，가이재안기화적태기재표면성공접지생물소，위태충식체표면생물화수식적연구제공신적방법화사로。
Objective To covalently immobilize biotin on the surface of the titanium substrate by biotin-avidin technology. Eventually, the study provides new experimental methods for titanium implant surface grafting bioactive molecules. Methods The surface of the titanium substrate was treated by 3-aminopropylphosphonic acid(APP), and enabled them to obtain free amino groups, which reacted with carboxyl groups to form amide bond to immobilize the biotin-avidin system on the surface of titanium implant by carbodiimide( EDC)-mediated. Results The samples were characterized by environmental scanning electron microscopy, fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and immunofluorescence, which demonstrated that biotin was successfully bonded to the titanium surface. Conclusion The biotin can be successfully immobilized on the surface of titanium implant with grafted amino groups through biotin-avidin technology. This study develops a new research approach and experimen-tal foundation of the biolization modification on the surface of titanium implant.