中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2015年
1期
22-27
,共6页
郭晶晶%俞丽云%黄美%梁荣喜%陈舜%何以敉%薛恩生
郭晶晶%俞麗雲%黃美%樑榮喜%陳舜%何以敉%薛恩生
곽정정%유려운%황미%량영희%진순%하이미%설은생
再灌注%精索扭转%细胞凋亡
再灌註%精索扭轉%細胞凋亡
재관주%정색뉴전%세포조망
reperfusion%Spermatic Cord torsion%apoptosis
目的:研究持续性低流量复灌注对睾丸不全扭转细胞凋亡的保护作用。方法实验分为轻度睾丸缺血组(A组)和中度睾丸缺血组(B组),两组各分为对照组(分别为AS组和BS组)和后处理组(A1和B1为45s维持组,A2和B2为45s渐增组,A3和B3为30s维持组,A4和B4为30s渐增组)。对照组进行直接复灌注,A1、B1组及A3、B3组分别进行45s和30s的维持相同的低流量复灌注,A2、B2组及A4、B4组分别进行45s和30s的渐增加的低流量复灌注。缺血前与术后3d进行睾丸超声造影,并检测睾丸组织Bcl-2相关X蛋白(Bax)、胱门蛋白酶-3(caspase-3)、凋亡指数(AI)。结果术后3d超声造影与缺血前比较,A4组及B4组的始增时间(AT)、达峰时间(TTP)、峰值强度(PI)、峰值基础强度差(PBD)差异比较均无统计学意义(P >0.05);峰值减半时间(DT/2)缩短,差异具统计学意义(P <0.05)。45s渐增组和30s组的AI明显低于对照组和45s维持组(P<0.01)。30s组Bax和caspase-3均显著低于对照组(P<0.01)。结论持续低流量复灌注对兔轻、中度睾丸不全扭转具有不同程度的保护作用,保护效果与复灌持续时间和血流增幅有关,以30s渐增低流量复灌保护作用最显著。
目的:研究持續性低流量複灌註對睪汍不全扭轉細胞凋亡的保護作用。方法實驗分為輕度睪汍缺血組(A組)和中度睪汍缺血組(B組),兩組各分為對照組(分彆為AS組和BS組)和後處理組(A1和B1為45s維持組,A2和B2為45s漸增組,A3和B3為30s維持組,A4和B4為30s漸增組)。對照組進行直接複灌註,A1、B1組及A3、B3組分彆進行45s和30s的維持相同的低流量複灌註,A2、B2組及A4、B4組分彆進行45s和30s的漸增加的低流量複灌註。缺血前與術後3d進行睪汍超聲造影,併檢測睪汍組織Bcl-2相關X蛋白(Bax)、胱門蛋白酶-3(caspase-3)、凋亡指數(AI)。結果術後3d超聲造影與缺血前比較,A4組及B4組的始增時間(AT)、達峰時間(TTP)、峰值彊度(PI)、峰值基礎彊度差(PBD)差異比較均無統計學意義(P >0.05);峰值減半時間(DT/2)縮短,差異具統計學意義(P <0.05)。45s漸增組和30s組的AI明顯低于對照組和45s維持組(P<0.01)。30s組Bax和caspase-3均顯著低于對照組(P<0.01)。結論持續低流量複灌註對兔輕、中度睪汍不全扭轉具有不同程度的保護作用,保護效果與複灌持續時間和血流增幅有關,以30s漸增低流量複灌保護作用最顯著。
목적:연구지속성저류량복관주대고환불전뉴전세포조망적보호작용。방법실험분위경도고환결혈조(A조)화중도고환결혈조(B조),량조각분위대조조(분별위AS조화BS조)화후처리조(A1화B1위45s유지조,A2화B2위45s점증조,A3화B3위30s유지조,A4화B4위30s점증조)。대조조진행직접복관주,A1、B1조급A3、B3조분별진행45s화30s적유지상동적저류량복관주,A2、B2조급A4、B4조분별진행45s화30s적점증가적저류량복관주。결혈전여술후3d진행고환초성조영,병검측고환조직Bcl-2상관X단백(Bax)、광문단백매-3(caspase-3)、조망지수(AI)。결과술후3d초성조영여결혈전비교,A4조급B4조적시증시간(AT)、체봉시간(TTP)、봉치강도(PI)、봉치기출강도차(PBD)차이비교균무통계학의의(P >0.05);봉치감반시간(DT/2)축단,차이구통계학의의(P <0.05)。45s점증조화30s조적AI명현저우대조조화45s유지조(P<0.01)。30s조Bax화caspase-3균현저저우대조조(P<0.01)。결론지속저류량복관주대토경、중도고환불전뉴전구유불동정도적보호작용,보호효과여복관지속시간화혈류증폭유관,이30s점증저류량복관보호작용최현저。
Objective Explore the protective effects of lasted low flow reperfusion postconditioning to incomplete testicular torsion apoptosis. Methods The experiment was randomly divided into mild ischemia group(A) and moderate ischemia group (B), and each group was divided into control groups (AS, BS) and postconditioning groups (A1, A2, A3, A4 and B1、B2、B3、B4). The control groups got reperfusion completely and immediately, groups A1, B1 and groups A3, B3 got same lasted low flow reperfusion with 45s and 30s, groups A2, B2 and groups A4, B4 got lasted gradually increasing low flow reperfusion with 45s and 30s. Got CEUS appearances before ischemia and postoperation, and analyzed the Bax, caspase-3 and AI of the affected testicle. Results Compared the postoperative parameters with preoperation, the AT, TTP, PI, PBD of the groups A4 and B4 had no change (P>0.05), the DT/2 were shorten(P<0.05). The AI of groups 2, 3, 4 were significantly lower than control group and group 1(P<0.01). The Bax and caspase-3 of postconditioning groups with 30s were significantly lower than control groups (P<0.01). Conclusion The lasted low flow reperfusion postconditioning has different protective effects to mild and moderate incomplete testicular torsion in rabbit, and the effect relates to the lasted time of reperfusion and the bloodflow growth, while the effect of lasted gradually increasing low flow reperfusion postconditioning with 30s is the most significant.