泰山医学院学报
泰山醫學院學報
태산의학원학보
JOURNAL OF TAISHAN MEDICAL COLLEGE
2015年
1期
3-6
,共4页
刘汝洋%高国芬%杜志华
劉汝洋%高國芬%杜誌華
류여양%고국분%두지화
鼻咽癌%血管生成,微血管密度%诱导型一氧化氮合酶%免疫组化
鼻嚥癌%血管生成,微血管密度%誘導型一氧化氮閤酶%免疫組化
비인암%혈관생성,미혈관밀도%유도형일양화담합매%면역조화
nasopharyngeal carcinoma%angiogenesis,MVD%iNOS%immunohistochemistry
目的探讨诱导型一氧化氮合酶（ inducible nitric oxide synthase，iNOS）在鼻咽癌中的表达与鼻咽癌生物学行为的关系，探讨肿瘤血管生成与鼻咽癌生物学行为的关系，探讨iNOS的表达与鼻咽癌血管生成的关系。方法<br> 采用非生物素二步免疫组化法，检测iNOS在鼻咽癌组织中表达，并根据CD34相关抗原标记血管内皮细胞。结果鼻咽癌组织中MVD显著高于慢性鼻咽炎组织（27.7±8.88 vs 9±3.1，P<0.05）。MVD与年龄、性别无关，临床分期Ⅲ、Ⅳ期组的MVD显著高于Ⅰ、Ⅱ期组（30.7±4.7 vs 22.2±7.5，P<0.05）。淋巴转移组MVD显著高于淋巴结未转移组（29.6±4.8 vs 20.5±4.8，P<0.05）。iNOS在鼻咽癌组织中阳性表达率为56.25%，iNOS的表达与年龄、性别无关。临床分期Ⅲ、Ⅳ期组iNOS的阳性表达率为70.97%，显著高于Ⅰ、Ⅱ期组29.41%（P<0.05）。淋巴转移组iNOS的阳性表达率为65.79%，显著高于淋巴结未转移组20.00%（P<0.05）。iNOS阳性表达组的MVD显著高于iNOS阴性表达组（30.0±5.4 vs 24.0±6.0，P<0.05）。结论血管生成及iNOS的表达与鼻咽癌生物学行为关系密切。iNOS表达促进了鼻咽癌血管生成及颈淋巴结的转移。测定MVD及iNOS的表达有助于判断鼻咽癌的恶性程度及预后。
目的探討誘導型一氧化氮閤酶（ inducible nitric oxide synthase，iNOS）在鼻嚥癌中的錶達與鼻嚥癌生物學行為的關繫，探討腫瘤血管生成與鼻嚥癌生物學行為的關繫，探討iNOS的錶達與鼻嚥癌血管生成的關繫。方法<br> 採用非生物素二步免疫組化法，檢測iNOS在鼻嚥癌組織中錶達，併根據CD34相關抗原標記血管內皮細胞。結果鼻嚥癌組織中MVD顯著高于慢性鼻嚥炎組織（27.7±8.88 vs 9±3.1，P<0.05）。MVD與年齡、性彆無關，臨床分期Ⅲ、Ⅳ期組的MVD顯著高于Ⅰ、Ⅱ期組（30.7±4.7 vs 22.2±7.5，P<0.05）。淋巴轉移組MVD顯著高于淋巴結未轉移組（29.6±4.8 vs 20.5±4.8，P<0.05）。iNOS在鼻嚥癌組織中暘性錶達率為56.25%，iNOS的錶達與年齡、性彆無關。臨床分期Ⅲ、Ⅳ期組iNOS的暘性錶達率為70.97%，顯著高于Ⅰ、Ⅱ期組29.41%（P<0.05）。淋巴轉移組iNOS的暘性錶達率為65.79%，顯著高于淋巴結未轉移組20.00%（P<0.05）。iNOS暘性錶達組的MVD顯著高于iNOS陰性錶達組（30.0±5.4 vs 24.0±6.0，P<0.05）。結論血管生成及iNOS的錶達與鼻嚥癌生物學行為關繫密切。iNOS錶達促進瞭鼻嚥癌血管生成及頸淋巴結的轉移。測定MVD及iNOS的錶達有助于判斷鼻嚥癌的噁性程度及預後。
목적탐토유도형일양화담합매（ inducible nitric oxide synthase，iNOS）재비인암중적표체여비인암생물학행위적관계，탐토종류혈관생성여비인암생물학행위적관계，탐토iNOS적표체여비인암혈관생성적관계。방법<br> 채용비생물소이보면역조화법，검측iNOS재비인암조직중표체，병근거CD34상관항원표기혈관내피세포。결과비인암조직중MVD현저고우만성비인염조직（27.7±8.88 vs 9±3.1，P<0.05）。MVD여년령、성별무관，림상분기Ⅲ、Ⅳ기조적MVD현저고우Ⅰ、Ⅱ기조（30.7±4.7 vs 22.2±7.5，P<0.05）。림파전이조MVD현저고우림파결미전이조（29.6±4.8 vs 20.5±4.8，P<0.05）。iNOS재비인암조직중양성표체솔위56.25%，iNOS적표체여년령、성별무관。림상분기Ⅲ、Ⅳ기조iNOS적양성표체솔위70.97%，현저고우Ⅰ、Ⅱ기조29.41%（P<0.05）。림파전이조iNOS적양성표체솔위65.79%，현저고우림파결미전이조20.00%（P<0.05）。iNOS양성표체조적MVD현저고우iNOS음성표체조（30.0±5.4 vs 24.0±6.0，P<0.05）。결론혈관생성급iNOS적표체여비인암생물학행위관계밀절。iNOS표체촉진료비인암혈관생성급경림파결적전이。측정MVD급iNOS적표체유조우판단비인암적악성정도급예후。
Objective:To explore the relationship between angiogenesis and biological behavior of nasopharyngeal carci-noma( NPC),the relationship between the expression of inducible nitric oxide synthase( iNOS)and biological behavior of NPC and the relationship between the expression of iNOS and angiogenesis of NPC. Methods:The expression of iNOS and micro vessel density( MVD)were assessed by immunohisto ± chemical methods in 48 specimens of NPC and in 13 speci-mens of chronic nasopharyngitis. The immuno-staining results and related chinical- pathological materials were analyzed by statistical methods. Results:MVD in NPC was significantly higher than that in chronic nasopharyngitis tissues(27. 7 ± 8. 88 vs 9 ± 3. 1,P<0. 05). MVD in NPC was not related to such factors as age and gender. MVD ofⅢandⅣstages was higher than that ofⅠandⅡstages(30. 7 ± 4. 7 vs 22. 2 ± 7. 5,P<0. 05). MVD of cases with lymphatic metastasis was sig-nificantly higher than that without lymphatic metastasis(29. 6 ± 4. 8 vs 20. 5 ± 4. 8,P<0. 05). The positive staining rate of iNOS was 56. 25%. The expression iNOS was not related to age and gender. The positive expression rate(70. 97%)of iN-OS in cases ofⅢ andⅣ stages was higher than that(29. 41%)in cases ofⅠandⅡstages(P<0. 05). The positive ex-pression rate(65. 79%)of iNOS in cases with lymphatic metastasis was higher than that(20. 00%)without lymphatic me-tastasis(P<0. 05). MVD in the cases with positive expression of iNOS was higher than in the cases with in the cases with negative expression(30 ± 5. 4 vs 24 ± 6. 0,P<0. 05). Conclusion:Angiogenesis of NPC is significantly related to the bio-logical behavior of NPC. The expression of iNOS is related to the biological behavior of NPC. The detection of MVD and the expression of iNOS in NPC can be used as indexes to determine the degree of malignancy and prognosis.
