CAJ | 학술논문

目的：研究缺氧诱导因子2α( HIF-2α)在肝细胞肝癌中的表达情况及其与血管生成和上皮间质转化( EMT)的关系。方法收集正常肝组织15例、肝癌组织和相应的癌旁组织各63例,采用免疫组化染色法检测HIF-2α的表达,分析其表达与肝癌临床病理特征及生存预后之间的关系；同时分析肝癌组织中HIF-2α、白细胞分化抗原34( CD34)、波形蛋白( Vimentin)的表达,探究 HIF-2α的表达与血管生成、EMT的关系。结果免疫组化染色法显示 HIF-2α在肝癌组织中主要在胞质表达,显著高于癌旁组织及正常肝组织( P<0．001)。 HIF-2α的表达与肿瘤最大径、肿瘤 TNM 分期、Edmondson分级及包膜和血管侵犯有关,与患者年龄、性别、乙肝表面抗原表达、甲胎蛋白( AFP)、Child-Pugh分级、肿瘤结节数目及肿瘤内坏死无关。相关性分析显示HIF-2α的表达与微血管密度(MVD)有关(t =5．919,P <0．001),与Vimentin的表达无关(r =1．013,P =0．209)。生存分析显示,高表达组术后总体生存率明显低于低表达组(P <0．001)。多因素分析显示瘤内坏死、肿瘤血管侵袭和 HIF-2α表达是影响肝癌患者总生存率的独立危险因素。结论HIF-2α可促进肝癌血管生成,但可能不参与EMT,可作为判断肝癌患者预后的指标之一。
목적：연구결양유도인자2α( HIF-2α)재간세포간암중적표체정황급기여혈관생성화상피간질전화( EMT)적관계。방법수집정상간조직15례、간암조직화상응적암방조직각63례,채용면역조화염색법검측HIF-2α적표체,분석기표체여간암림상병리특정급생존예후지간적관계；동시분석간암조직중HIF-2α、백세포분화항원34( CD34)、파형단백( Vimentin)적표체,탐구 HIF-2α적표체여혈관생성、EMT적관계。결과면역조화염색법현시 HIF-2α재간암조직중주요재포질표체,현저고우암방조직급정상간조직( P<0．001)。 HIF-2α적표체여종류최대경、종류 TNM 분기、Edmondson분급급포막화혈관침범유관,여환자년령、성별、을간표면항원표체、갑태단백( AFP)、Child-Pugh분급、종류결절수목급종류내배사무관。상관성분석현시HIF-2α적표체여미혈관밀도(MVD)유관(t =5．919,P <0．001),여Vimentin적표체무관(r =1．013,P =0．209)。생존분석현시,고표체조술후총체생존솔명현저우저표체조(P <0．001)。다인소분석현시류내배사、종류혈관침습화 HIF-2α표체시영향간암환자총생존솔적독립위험인소。결론HIF-2α가촉진간암혈관생성,단가능불삼여EMT,가작위판단간암환자예후적지표지일。
Objective To explore the expression of hypoxia-inducible factor-2α ( HIF-2α) in hepatocellular carci-noma and the relationship with epithelial-mesenchymal transition(EMT)and angiogenesis. Methods The expres-sions of HIF-2αin 63 cases of hepatocellular carcinoma tumor tissues and 15 normal liver tissues were examined by immunohistochemistry. To assess the relationship between the expressions of HIF-2α, EMT and angiogenesis,the expressions of CD34 and Vimentin were detected in hepatocellular carcinoma. Results Immunohistochemistry showed that HIF-2α mainly expressed in the cytoplasm, the rate of HIF-2α overexpression in hepatocellular carci-noma tissues was significantly higher than the other two groups ( P<0. 001 ) . The expression of HIF-2αwas correla-ted with tumor size,TNM stages,Edmondson grade, capsule or vascular invasion. HIF-2α expression had a signifi-cantly positive correlation with microvascular density ( t=5. 919 , P<0. 001 ) , but not vimentin ( r=1. 013 , P=0. 209 ) . Survival analysis showed that the prognosis was poor in patients with HIF-2αoverexpression ( P<0. 001 ) . Cox regression demonstrated that the expression of HIF-2α,tumor necrosis and vascular invasion were the independ-ent risk factors for cumulative survival in hepatocellular carcinoma patients. Conclusion HIF-2α can promote an-giogenesis, but may not participate in EMT. It may be a potential tumor marker for prognosis of hepatocellular car-cinoma.