CAJ | 학술논문

目的：观察血清中血管生成素-1( Ang-1)、血管生成素-2(Ang-2)及其受体Tie2与血管内皮生长因子(VEGF)在糖尿病肾病( DN)病情进展中的水平变化规律,探讨平衡其比率在DN中的重要性。方法选取2型糖尿病患者60例,依据尿白蛋白排泄率( UAER)分为3组：正常白蛋白尿患者20例( DM1组)、微量白蛋白尿患者22例( DM2组)、大量白蛋白尿患者18例( DM3组),另设健康对照患者22例( NC 组)。采用 ELISA 法检测受试者血清 Ang-1、Ang-2、Tie2、VEGF水平,同时检测相关生化指标。结果① DM1组血清VEGF、Ang-2水平高于NC组, DM2组、DM3组进一步升高,4组间差异均有统计学意义( P<0．05)。② DM1组血清Ang-1水平高于NC组,DM2组、DM3组逐渐下降,4组间差异无统计学意义。③ Tie2水平差异无统计学意义。④UAER与Ang-2、VEGF 呈正相关性( r =0．470、0．404, P <0．01),与Ang-1呈负相关性(r=-0．310,P<0．05)； Ang-2与VEGF、Tie2呈正相关性(r=0．450、0．725,P<0．01)；Ang-1与Tie2呈正相关性(r=0．571,P<0．01),与Ang-2、VEGF呈负相关性(r=-0．497、-0．396,P<0．01,P<0．05)。⑤以UAER为应变量行多元线性逐步回归分析显示：Ang-1、Ang-2、VEGF、肌酐进入回归方程,是影响UAER的独立相关因子。结论血清中 Ang-1、Ang-2、Tie2、VEGF 水平与 DN密切相关,可能参与了DN的发生发展,并影响DN病情的进程。检测血清Ang/Tie2及VEGF可作为评估DN病情的重要指标。
목적：관찰혈청중혈관생성소-1( Ang-1)、혈관생성소-2(Ang-2)급기수체Tie2여혈관내피생장인자(VEGF)재당뇨병신병( DN)병정진전중적수평변화규률,탐토평형기비솔재DN중적중요성。방법선취2형당뇨병환자60례,의거뇨백단백배설솔( UAER)분위3조：정상백단백뇨환자20례( DM1조)、미량백단백뇨환자22례( DM2조)、대량백단백뇨환자18례( DM3조),령설건강대조환자22례( NC 조)。채용 ELISA 법검측수시자혈청 Ang-1、Ang-2、Tie2、VEGF수평,동시검측상관생화지표。결과① DM1조혈청VEGF、Ang-2수평고우NC조, DM2조、DM3조진일보승고,4조간차이균유통계학의의( P<0．05)。② DM1조혈청Ang-1수평고우NC조,DM2조、DM3조축점하강,4조간차이무통계학의의。③ Tie2수평차이무통계학의의。④UAER여Ang-2、VEGF 정정상관성( r =0．470、0．404, P <0．01),여Ang-1정부상관성(r=-0．310,P<0．05)； Ang-2여VEGF、Tie2정정상관성(r=0．450、0．725,P<0．01)；Ang-1여Tie2정정상관성(r=0．571,P<0．01),여Ang-2、VEGF정부상관성(r=-0．497、-0．396,P<0．01,P<0．05)。⑤이UAER위응변량행다원선성축보회귀분석현시：Ang-1、Ang-2、VEGF、기항진입회귀방정,시영향UAER적독립상관인자。결론혈청중 Ang-1、Ang-2、Tie2、VEGF 수평여 DN밀절상관,가능삼여료DN적발생발전,병영향DN병정적진정。검측혈청Ang/Tie2급VEGF가작위평고DN병정적중요지표。
Objective To observe the regular levels change of serum angiopoietin-1 (Ang-1),angiopoietin-2 (Ang-2) and receptor Tie2 as well as vascular endothelia growth factor (VEGF) in the progerssion of diabetic nephropa-thy ( DN) , and investigate the importance of ratio of balance among them in DN. Methods Sixty Chinese patients with type 2 diabetes were enrolled. According to their UAER diabetic patients were divided into three groups ran-domly:20 patients with normoalbuminuric diabetic ( DM1 group) , 22 with microalbuminuric diabetic( DM2 group) and 18 proteinuric diabetic (DM3 group). A total of 22 health contros were selected(NC group). Serum Ang-1, Ang-2,Tie2,VEGF levels were detected by ELISA. At the same time, related biochemical indicators were tested. Results ①The level of serum Ang-2,VEGF increased in DM1 compared with NC, and serum Ang-2,VEGF levels in DM2 and DM3 were much higher. There was statistical difference among four groups (P<0. 05). ②The level of serum Ang-1 increased in DM1 compared with NC, but serum Ang-1 levels in DM2 and DM3 were much lower.③ There was no statistical significance in difference among the four groups. There was no statistical significance in differences of Tie2 .④ UAER had positive correlation with serum Ang-2 , VEGF ( r =0. 470 , 0. 404 , P <0. 01 ) . UAER had negative correlation with serum Ang-1(r= -0. 310,P<0. 05). Ang-2 had positive correlation with ser-um VEGF,Tie2(r =0. 450,0. 725,P <0. 01). Ang-1 had positive correlation with serum Tie2(r =0. 571,P <0. 01). Ang-1 had negative correlation with serum Ang-2,VEGF (r= -0. 497,-0. 396,P<0. 01,P<0. 05). ⑤Line to UAER level as the dependent variable, multiple stepwise regression analysis showed that: Ang-1,Ang-2, VEGF,creatinine entered regression equation. They were independent correlation factors for UAER. Conclusion Serum Ang-1,Ang-2,Tie2 and VEGF levels are closely related with DN. They may participate in the development of DN and affect the process of DN. Detection of serum Ang/Tie2 and VEGF levels can be used as a reference in-dex for the condition of DN.