CAJ | 학술논문

目的：：探讨2型糖尿病大鼠肝脏肝细胞核因子-4α(HNF-4α)表达的变化和意义。方法：24只雄性SD大鼠随机分为正常对照组和糖尿病模型组，每组12只大鼠。链脲佐菌素连续腹腔注射法制作2型糖尿病大鼠模型。采用SP免疫组织化学染色、Western Blotting法、RT-PCR法检测大鼠肝脏HNF-4α的表达情况。结果：与正常对照组大鼠比较，糖尿病模型组大鼠肝脏HNF-4α蛋白和mRNA的表达均明显升高(P＜0.01)。结论：肝脏HNF-4α表达的升高可能参与了2型糖尿病时肝脏损伤的发生发展。
목적：：탐토2형당뇨병대서간장간세포핵인자-4α(HNF-4α)표체적변화화의의。방법：24지웅성SD대서수궤분위정상대조조화당뇨병모형조，매조12지대서。련뇨좌균소련속복강주사법제작2형당뇨병대서모형。채용SP면역조직화학염색、Western Blotting법、RT-PCR법검측대서간장HNF-4α적표체정황。결과：여정상대조조대서비교，당뇨병모형조대서간장HNF-4α단백화mRNA적표체균명현승고(P＜0.01)。결론：간장HNF-4α표체적승고가능삼여료2형당뇨병시간장손상적발생발전。
[ABSTRACT]Objective:To investigate changes and signiifcance of hepatocyte nuclear factor 4 alpha (HNF-4α) in liver of type 2 diabetes mellitus rats model.Methods:24 male SD rats were randomly divided into normal control group and diabetes model group with 12 rats in each group. The type 2diabetes model rats were induced by continuous intraperitoneal injection of streptozotocin. SP immunohistochemical stain, Western Blotting and RT-PCR were used to detect HNF-4αexpression in liver.Results: Compared with rats in normal control group, the HNF-4α protein and mRNA expression in liver of rats in diabetes model group increased obviously (P<0.01).Conclusions: High expression of HNF-4α may participate development of liver injury during diabetes mellitus.