CAJ | 학술논문

目的：探讨免疫组化CK、P63与原位杂交EBER在诊断复发性鼻咽癌的应用。方法收集54例临床怀疑鼻咽癌复发病例，分别进行CK、P63及原位杂交EBER检测。结果54例中有40例为复发癌，14例为炎症或放疗后改变。40例复发癌CK、P63均阳性（100％），38例EBER阳性（95％），而14例非复发癌CK、P63及EBER均为阴性（100％），两组阳性率对比有统计学差异（P＜0．05）。40例复发癌中2例为角化型鳞癌（5％），非角化型分化性癌4例（10％），非角化型未分化性癌34例（85％），且复发组织学类型与原发癌类型一致。结论结合形态学，免疫组化CK、P63及原位杂交EBER，可以对是否为鼻咽癌复发作出明确诊断。
목적：탐토면역조화CK、P63여원위잡교EBER재진단복발성비인암적응용。방법수집54례림상부의비인암복발병례，분별진행CK、P63급원위잡교EBER검측。결과54례중유40례위복발암，14례위염증혹방료후개변。40례복발암CK、P63균양성（100％），38례EBER양성（95％），이14례비복발암CK、P63급EBER균위음성（100％），량조양성솔대비유통계학차이（P＜0．05）。40례복발암중2례위각화형린암（5％），비각화형분화성암4례（10％），비각화형미분화성암34례（85％），차복발조직학류형여원발암류형일치。결론결합형태학，면역조화CK、P63급원위잡교EBER，가이대시부위비인암복발작출명학진단。
Objective To investigate application of Cytokeratin ( CK ) , p63 and Epstein-Barr virus-encoded small RNAs( EBER) by immunohistochemistry staining and in situ hybridization in diagnosis of recurrent nasopharyngeal carcina( rNPC) .Methods By collecting the clinical suspicion of 54 cases of rNPC, to CK,P63 and in situ hybridization EBER detection respectively.Results 40 cases were recurrence of cancer, 14 cases of inflammation or change after radiotherapy.40 cases of rNPC CK and P63 were positive ( 100%) , 38 cases of EBER positive (95%),14 cases of non recurrent cancer CK, P63 and EBER were negative (100%).The two groups had statistical difference between the positive rate of contrast (P <0.05).There were 2 cases of keratinizing squamous cell carcinoma(5%) in 40 cases of recurrent carcinoma .Non cornification differentiated carcinoma in 4 cases ( 10%) , type non cornification undifferentiated carcinoma with 34 cases ( 85%) .and the Recurrent carcinoma histological types and primary cancer types are the same.Conclusions Combined with the morphological, immunohistochemical CK, P63 and EBER in situ hybridization, can clear diagnosis to make whether for recurrent nasopharyngeal carcinoma.