齐齐哈尔医学院学报
齊齊哈爾醫學院學報
제제합이의학원학보
JOURNAL OF QIQIHAR MEDICAL COLLEGE
2015年
6期
784-786
,共3页
脊髓损伤%雪旺细胞%运动终板%乙酰胆碱酯酶%降钙素基因相关肽
脊髓損傷%雪旺細胞%運動終闆%乙酰膽堿酯酶%降鈣素基因相關肽
척수손상%설왕세포%운동종판%을선담감지매%강개소기인상관태
Spinal cord injury%Schwann cells%Motor end plate%AChE%CGSP
目的：观察脊髓损伤后雪旺细胞移植对运动终板的保护作用。方法培养纯化并S100鉴定雪旺细胞。70只Wistar大鼠分别分为对照组、单纯损伤组、损伤加移植细胞组，移植细胞时间分别为伤后1小时、4小时、8小时、12小时、24小时，每组分别伤后1周、4周及8周取材。评定细胞移植后存活率及测定脊髓组织LDH值，并对腓肠肌运动终板行AChE和CGRP免疫组化染色，测定表达量。结果雪旺细胞第4代时纯度为95．3％，移植不同时间存活率无统计学差异（P＞0．05），早期移植雪旺细胞LDH值有明显下降并且伤后8～12小时为早期时间窗，损伤加移植细胞组相比单纯损伤组在伤后1周、4周及8周AChE及CGSP组织化学显示运动终板染色较深（P＜0．05）。结论雪旺细胞早期移植可以保护脊髓损伤后运动终板，可能通过减少LDH的释放起作用。
目的：觀察脊髓損傷後雪旺細胞移植對運動終闆的保護作用。方法培養純化併S100鑒定雪旺細胞。70隻Wistar大鼠分彆分為對照組、單純損傷組、損傷加移植細胞組，移植細胞時間分彆為傷後1小時、4小時、8小時、12小時、24小時，每組分彆傷後1週、4週及8週取材。評定細胞移植後存活率及測定脊髓組織LDH值，併對腓腸肌運動終闆行AChE和CGRP免疫組化染色，測定錶達量。結果雪旺細胞第4代時純度為95．3％，移植不同時間存活率無統計學差異（P＞0．05），早期移植雪旺細胞LDH值有明顯下降併且傷後8～12小時為早期時間窗，損傷加移植細胞組相比單純損傷組在傷後1週、4週及8週AChE及CGSP組織化學顯示運動終闆染色較深（P＜0．05）。結論雪旺細胞早期移植可以保護脊髓損傷後運動終闆，可能通過減少LDH的釋放起作用。
목적：관찰척수손상후설왕세포이식대운동종판적보호작용。방법배양순화병S100감정설왕세포。70지Wistar대서분별분위대조조、단순손상조、손상가이식세포조，이식세포시간분별위상후1소시、4소시、8소시、12소시、24소시，매조분별상후1주、4주급8주취재。평정세포이식후존활솔급측정척수조직LDH치，병대비장기운동종판행AChE화CGRP면역조화염색，측정표체량。결과설왕세포제4대시순도위95．3％，이식불동시간존활솔무통계학차이（P＞0．05），조기이식설왕세포LDH치유명현하강병차상후8～12소시위조기시간창，손상가이식세포조상비단순손상조재상후1주、4주급8주AChE급CGSP조직화학현시운동종판염색교심（P＜0．05）。결론설왕세포조기이식가이보호척수손상후운동종판，가능통과감소LDH적석방기작용。
Objective To observe protection effect of Schwann cells to motor end plate after spinal cord injury.Methods Schwann cells were cultured, purified and identified with S100.Seventy Wistar rats were divided into control group, damage group, damage and cell transplantation group.The time points of cell transplantation were 1 hour, 4 hours ,8 hours, 12 hours, 24 hours after injury, respectively.Specimens were taken 1 week, 4 weeks and 8 weeks after injury from each group.Then we evaluated survival rate of transplanted cells and determinate LDH values of the spinal cord tissue.AChE and CGRP immunohistochemical staining of the motor end plate of the gastrocnemius muscle was performed to measure their expression.Results The purity of Schwann cells of the 4th generation was 95.3%, and transplant survival rate for different time showed no statistical difference (P>0.05).LDH values declined obviously when transplantation of Schwann cells at early stage and 8 to 12 hours after injury can be seen as early time window.Injury and cell transplantation group compared with the only damage group in 1 week, 4 weeks and 8 weeks after injury had deeper dye in motor endplate by doing AChE and CGSP histochemistry (P<0.05).Conclusions Early transplantion of schwann cells can protect the motor end plate after spinal cord injury, which may work by reducing the release of LDH.
