CAJ | 학술논문

目的：探讨层流剪切力( LSS)对间充质干细胞( MSCs)增殖和凋亡的影响。方法选择间充质干细胞( MSCs)，并对其进行体外流体剪切力力学加载(生理范围内)；通过DNA合成了解增殖情况，采用流式细胞仪及PCR技术检测其凋亡情况。结果在生理范围内的流体力学加载，细胞增殖与其加载力相关；该范围内的力学刺激对MSCs的凋亡起抑制作用。结论生理范围内的流体剪切力诱导MSCs进入休眠状态。
목적：탐토층류전절력( LSS)대간충질간세포( MSCs)증식화조망적영향。방법선택간충질간세포( MSCs)，병대기진행체외류체전절력역학가재(생리범위내)；통과DNA합성료해증식정황，채용류식세포의급PCR기술검측기조망정황。결과재생리범위내적류체역학가재，세포증식여기가재력상관；해범위내적역학자격대MSCs적조망기억제작용。결론생리범위내적류체전절력유도MSCs진입휴면상태。
Objective To investigate the effects of laminar shear stress ( LSS) on the proliferation and apoptosis of mesenchymal stem cells ( MSCs) . Methods The influences of laminar shear stress ( the physiological levels) on MSCs were studied. DNA synthesis and cell cycle were measured,to detect the cellular proliferation. The apoptosis-related gene expression ( Bcl-2/Bax) was examined to determine the effect of LSS. Results The percentage of MSCs’ proliferation rate was reduced by the fluid shear stress. Furthermore,it was detected that LSS exerted a potent suppression effect on MSC apoptosis. Conclusion These data revealed a critical role of LSS in maintaining the quies-cence of MSCs.