CAJ | 학술논문

目的应用ZD7288特异性阻断超极化激活环核苷酸门控阳离子非选择性通道( HCN通道)后观察人输尿管离体平滑肌肌条自发性收缩活动的变化，从功能学上初步探讨HCN通道在输尿管平滑肌自发性收缩中的作用。方法收集临床上因肾肿瘤或囊肿等行肾切除手术患者的离体输尿管标本12例，经确认患者手术前均未接受放疗、化疗或免疫治疗等特殊处理，进行输尿管离体平滑肌肌条实验，观察在ZD7288作用下输尿管离体平滑肌肌条的收缩幅度和频率的变化。以人肠道组织为阳性对照，剩余标本用于检测HCN通道在人输尿管上的表达情况。结果经RT-PCR、WB法( Western blot)技术及免疫组化法检测，发现正常人输尿管存在HCN通道的表达，且HCN通道主要表达于人输尿管的黏膜层与平滑肌层；离体肌条实验中加入ZD7288后，肌条的收缩频率明显变慢，收缩幅度变化不明显，自身前后对比有统计学差异(P<0.01)。结论 HCN通道可能在人输尿管自发性收缩中发挥着重要作用。
목적응용ZD7288특이성조단초겁화격활배핵감산문공양리자비선택성통도( HCN통도)후관찰인수뇨관리체평활기기조자발성수축활동적변화，종공능학상초보탐토HCN통도재수뇨관평활기자발성수축중적작용。방법수집림상상인신종류혹낭종등행신절제수술환자적리체수뇨관표본12례，경학인환자수술전균미접수방료、화료혹면역치료등특수처리，진행수뇨관리체평활기기조실험，관찰재ZD7288작용하수뇨관리체평활기기조적수축폭도화빈솔적변화。이인장도조직위양성대조，잉여표본용우검측HCN통도재인수뇨관상적표체정황。결과경RT-PCR、WB법( Western blot)기술급면역조화법검측，발현정상인수뇨관존재HCN통도적표체，차HCN통도주요표체우인수뇨관적점막층여평활기층；리체기조실험중가입ZD7288후，기조적수축빈솔명현변만，수축폭도변화불명현，자신전후대비유통계학차이(P<0.01)。결론 HCN통도가능재인수뇨관자발성수축중발휘착중요작용。
Objective To investigate effects of hyperpolarization-activated cyclic nucleotide-gated cation nonselective channel in the hu-man ureter on the spontaneous contraction of smooth muscles. Methods Four HCN subtypes were detected in human ureteral tissue using reverse transcription polymerase chain reaction,Western blotting and immunohistochemical. ZD7288,the HCN blocker, was used to observe the changes of ureteral muscle contraction amplitude and frequency by applying the ureteral smooth muscle strip test in vitro. Results HCN1-4 isoforms were all identified in human ureter using reverse transcription-polymerase chain reaction and Western blotting. Through the immunohistochemical,HCN channel was found mostly in the urothelium layer and muscular layer of human ureteral wall. ZD7288 significantly decreased the bladder excitation. Conclusion All 4 HCN channel hypotypes exist in the human ureter, and affect the ureteral excitation.