医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2015年
14期
84-85
,共2页
血氨测定%抗凝剂%肝素%EDTA
血氨測定%抗凝劑%肝素%EDTA
혈안측정%항응제%간소%EDTA
Ammonia determination%Anticoagulant%Heparin%EDTA
目的探讨肝素和EDTA抗凝血对血氨的检测结果是否存在差异。方法选取2013年1月~9月在大连医科大学附属第二医院就诊的患者90例根据血氨参考范围入选病例分成Ⅰ、Ⅱ、Ⅲ三组，每组30例，分别采其静脉血P<3 ml，注入肝素抗凝、EDTA抗凝两种不同真空采集管中，经离心分离后即提取血浆用干化学法进行血氨值测定并计算均值和差值，进行统计学分析，比较两组均数差别有无统计学意义，采用检验。结果Ⅰ组肝素抗凝血值为（22.23±6.19）μmol/L，EDTA抗凝血值为（40.5±15.68）μmol/L，两组相比较=5.94，<0.01；Ⅱ组肝素抗凝血值为（50.79±13.68）μmol/L，EDTA抗凝血值为（74.35±20.9）μmol/L，两组相比较，=5.17，<0.01；芋组肝素抗凝血值为（121.59±24.72）μmol/L，EDTA抗凝血值为（156.83±21.82）μmol/L，两组相比较，=5.85，<0.01。结论不同血样采集管对干化学法血氨测定结果有一定影响，肝素抗凝组的测定结果低于EDTA抗凝组，有显著性差异（<0.01）。由于本实验还受到环境温度、标本放置时间等因素的影响，而肝素的稳定性较高。因此，为提高测定结果的准确性，建议临床血氨测定使用肝素抗凝真空管采集标本。
目的探討肝素和EDTA抗凝血對血氨的檢測結果是否存在差異。方法選取2013年1月~9月在大連醫科大學附屬第二醫院就診的患者90例根據血氨參攷範圍入選病例分成Ⅰ、Ⅱ、Ⅲ三組，每組30例，分彆採其靜脈血P<3 ml，註入肝素抗凝、EDTA抗凝兩種不同真空採集管中，經離心分離後即提取血漿用榦化學法進行血氨值測定併計算均值和差值，進行統計學分析，比較兩組均數差彆有無統計學意義，採用檢驗。結果Ⅰ組肝素抗凝血值為（22.23±6.19）μmol/L，EDTA抗凝血值為（40.5±15.68）μmol/L，兩組相比較=5.94，<0.01；Ⅱ組肝素抗凝血值為（50.79±13.68）μmol/L，EDTA抗凝血值為（74.35±20.9）μmol/L，兩組相比較，=5.17，<0.01；芋組肝素抗凝血值為（121.59±24.72）μmol/L，EDTA抗凝血值為（156.83±21.82）μmol/L，兩組相比較，=5.85，<0.01。結論不同血樣採集管對榦化學法血氨測定結果有一定影響，肝素抗凝組的測定結果低于EDTA抗凝組，有顯著性差異（<0.01）。由于本實驗還受到環境溫度、標本放置時間等因素的影響，而肝素的穩定性較高。因此，為提高測定結果的準確性，建議臨床血氨測定使用肝素抗凝真空管採集標本。
목적탐토간소화EDTA항응혈대혈안적검측결과시부존재차이。방법선취2013년1월~9월재대련의과대학부속제이의원취진적환자90례근거혈안삼고범위입선병례분성Ⅰ、Ⅱ、Ⅲ삼조，매조30례，분별채기정맥혈P<3 ml，주입간소항응、EDTA항응량충불동진공채집관중，경리심분리후즉제취혈장용간화학법진행혈안치측정병계산균치화차치，진행통계학분석，비교량조균수차별유무통계학의의，채용검험。결과Ⅰ조간소항응혈치위（22.23±6.19）μmol/L，EDTA항응혈치위（40.5±15.68）μmol/L，량조상비교=5.94，<0.01；Ⅱ조간소항응혈치위（50.79±13.68）μmol/L，EDTA항응혈치위（74.35±20.9）μmol/L，량조상비교，=5.17，<0.01；우조간소항응혈치위（121.59±24.72）μmol/L，EDTA항응혈치위（156.83±21.82）μmol/L，량조상비교，=5.85，<0.01。결론불동혈양채집관대간화학법혈안측정결과유일정영향，간소항응조적측정결과저우EDTA항응조，유현저성차이（<0.01）。유우본실험환수도배경온도、표본방치시간등인소적영향，이간소적은정성교고。인차，위제고측정결과적준학성，건의림상혈안측정사용간소항응진공관채집표본。
Objective To study the heparin and EDTA anticoagulant blood for blood ammonia detection results whether there are dif erences. Methods According to blood ammonia reference range selected cases into Ⅰ, II, Ⅲ three groups,each group of 30 patients, 90 patients. Respectively adopt its venous blood about 3 ml, inject heparin anticoagulation, EDTA anticoagulation two dif erent vacuum acquisition tube, the centrifugal separation namely after extraction plasma or serum with dry chemical method for blood ammonia value determination and calculation mean and dif erence, car ies on the statistical analysis, and compared between the two groups have statistical significance dif erence mean, the t test. ResultsⅠgroup heparin anticoagulation group value was 22.23±6.19(μmol/L), EDTA anticoagulation group value was 40.5±15.68 (μ mol/L), two groups of compared = 5.94, < 0.01; II group heparin anticoagulation group value was 50.79±13.68 (μ mol/L), EDTA anticoagulation group value was 74.35±20.9 (μ mol/L), two groups of phase comparison, = 5.17, < 0.01; Ⅲ group heparin anticoagulation group value was 121.59±24.72 (μ mol/L),EDTA anticoagulation group value was 156.83±21.82 (μ mol/L), two groups of phase comparison, = 5.85, < 0.01. Conclusion Dif erent blood col ection tube to dry chemical method blood ammonia determination results have a certain influence, heparin anticoagulation group of the determination results below EDTA anticoagulation group, there is significantly statistical dif erence ( < 0.01). Because of this experiment is influenced by environmental temperature, specimen placed time and so on factors, and the stability of the heparin higher. Therefore, in order to improve the veracity of test results, it is suggested that clinical blood ammonia determination using heparin anticoagulation vacuum tube specimen col ection.
