CAJ | 학술논문

目的：探讨Fas及Fas-L对小梁切除术后成纤维细胞凋亡的影响。方法：选取健康白兔20只，随机分为对照组和观察组，每组各10只，分别切下两组白兔带结膜、筋膜、巩膜及角膜缘的滤过手术区组织，进行成纤维细胞的培养，其中观察组进行青光眼小梁切除术。随机选取两组中各5例成纤维细胞进行FasMcAb处理，使用PI染色、流式细胞仪检测所有细胞凋亡率。结果：经FasMcAb处理的正常成纤维细胞凋亡率远高于未经FasMcAb处理的正常成纤维细胞，两组比较差异具有统计学意义（P<0.01）；经FasMcAb处理的青光眼小梁切除术滤过区组织成纤维细胞凋亡率远高于未经FasMcAb处理的青光眼小梁切除术滤过区组织成纤维细胞，两组比较差异具有统计学意义（P<0.01）；经FasMcAb处理的青光眼小梁切除术滤过区组织成纤维细胞凋亡率大于正常成纤维细胞，两组比较差异具有统计学意义（P<0.01）。结论：Fas/Fas-L能够加速小梁切除术后成纤维细胞凋亡，可为减少青光眼小梁切除术后瘢痕化寻找一个新的突破口。
목적：탐토Fas급Fas-L대소량절제술후성섬유세포조망적영향。방법：선취건강백토20지，수궤분위대조조화관찰조，매조각10지，분별절하량조백토대결막、근막、공막급각막연적려과수술구조직，진행성섬유세포적배양，기중관찰조진행청광안소량절제술。수궤선취량조중각5례성섬유세포진행FasMcAb처리，사용PI염색、류식세포의검측소유세포조망솔。결과：경FasMcAb처리적정상성섬유세포조망솔원고우미경FasMcAb처리적정상성섬유세포，량조비교차이구유통계학의의（P<0.01）；경FasMcAb처리적청광안소량절제술려과구조직성섬유세포조망솔원고우미경FasMcAb처리적청광안소량절제술려과구조직성섬유세포，량조비교차이구유통계학의의（P<0.01）；경FasMcAb처리적청광안소량절제술려과구조직성섬유세포조망솔대우정상성섬유세포，량조비교차이구유통계학의의（P<0.01）。결론：Fas/Fas-L능구가속소량절제술후성섬유세포조망，가위감소청광안소량절제술후반흔화심조일개신적돌파구。
Objective: To explore apoptosis of the fibroblast induced by Fas and Fas-L after trabeculectomy. Method: A total of 20 healthy white rabbits were randomly divided into the control group and observation group,10 cases in each group,and the rabbits in the observation group were given glaucoma trabeculectomy.Two groups of tissues in trabeculectomy filtration area, which contain conjunctiva, anadesma, sclera and limbus corneae were cut down, and the fibroblasts of the tissues were cultivated. 5 cases of fibroblasts in each group were randomly selected and treated by FasMcAb. At last, PI staining and flow cytometry were used for testing the apoptotic rate of all the fibroblasts.Result:The apoptotic rate of normal fibroblasts induced by FasMcAb was higher than that of normal fibroblasts without induced by FasMcAb, the difference was statistically significant(P<0.01); the apoptotic rate of fibroblasts from tissues in trabeculectomy filtration area induced by FasMcAb was higher than that of fibroblasts from tissues in trabeculectomy filtration area without induced by FasMcAb,the difference was statistically significant(P<0.01);the effect of FasMcAb on the apoptotic rate of fibroblasts from tissues in trabeculectomy filtration area was more significant than that on normal fibroblasts, the difference was statistically significant(P<0.01).Conclusion:Fas/Fas-L can accelerate apoptosis of the fibroblast after trabeculectomy, and helps to find a new breakthrough to reduce the scar after glaucoma trabeculectomy.