CAJ | 학술논문

目的：观察幼年小鼠与成年小鼠海马齿状回中Hes1的表达差异，初步分析其对成年神经再生的影响。方法：将C57BL/6雄性小鼠分为幼年组（10 d，N组）和成年组（4个月，A组），每组8只。分别对Hes1、BrdU、Doublecortin（DCX）、NeuN采用免疫荧光技术染色并进行相应的细胞计数，初步分析BrdU、Hes1双阳性细胞的细胞类型及表达水平。在显微镜下分离海马齿状回，采用Western blot检测Hes1的蛋白水平，观察对比两组间Hes1蛋白表达的差异。结果：（1）Western blot检测中A组中的Hes1蛋白的表达水平明显高于N组，两组比较差异有统计学意义（P<0.01）。（2）Hes1（+）细胞主要分布于齿状回颗粒细胞下层，并且大部分Hes1阳性细胞同时表达Brdu；（3）A组Hes1（+）细胞数量明显多于N组，而BrdU（+）细胞明显少于N组，且差异均有统计学意义（P<0.05）。结论：Hes1在幼年小鼠与成年小鼠齿状回颗粒细胞下层中的表达差异，导致了神经细胞增殖水平的不同，表明Hes1的高表达可能抑制了神经再生。
목적：관찰유년소서여성년소서해마치상회중Hes1적표체차이，초보분석기대성년신경재생적영향。방법：장C57BL/6웅성소서분위유년조（10 d，N조）화성년조（4개월，A조），매조8지。분별대Hes1、BrdU、Doublecortin（DCX）、NeuN채용면역형광기술염색병진행상응적세포계수，초보분석BrdU、Hes1쌍양성세포적세포류형급표체수평。재현미경하분리해마치상회，채용Western blot검측Hes1적단백수평，관찰대비량조간Hes1단백표체적차이。결과：（1）Western blot검측중A조중적Hes1단백적표체수평명현고우N조，량조비교차이유통계학의의（P<0.01）。（2）Hes1（+）세포주요분포우치상회과립세포하층，병차대부분Hes1양성세포동시표체Brdu；（3）A조Hes1（+）세포수량명현다우N조，이BrdU（+）세포명현소우N조，차차이균유통계학의의（P<0.05）。결론：Hes1재유년소서여성년소서치상회과립세포하층중적표체차이，도치료신경세포증식수평적불동，표명Hes1적고표체가능억제료신경재생。
Objective:To investigate the effect of Hes1 on adult neurogensis from the level of neurogenesis and Hes1 expression in dentate gyrus of hippocampus between neonatal mice and adult mice.Method:The C57BL/6 male mice were separated into two groups:the newborn mice was as control for neonatal group(10 days old,N group),and the adult mice (4 months old, A group) were assigned to adult group,8 mice in each group.The immunofluorescence staining of Hes1,Brdu,Doublecortin (DCX) and NeuN were performed and the cell count was corresponding recorded, double positive cells types and level of expression were preliminary analyzed of Brdu,Hes1.The hippocampal dentate gyrus was separated in microscope,Hes1 protein levels were detected by Western blot, the differences of two groups’Hes1 protein expression levels were observed and compared.Result:(1)Hes1 protein levels of A group was hingher than the N group from Western blot detection,the difference was statistically significant(P<0.05);(2)Hes1 positive cells mainly distributed in the dentate gyrus granular cell lower, and most Hes1 positive cells expressed Brdu at the same time;(3)the group A of Hes1(+) cells significantly was more than N group, and BrdU(+) obviously less than N group,the difference was statistically significant(P<0.05).Conclusion:The different expression of Hes1 from neonatal mice and adult mice in dentate gyrus of hippocampus,lead to different neurons generated levels, indicating high expression of Hes1 may inhibit neurogenesis.