黑龙江八一农垦大学学报
黑龍江八一農墾大學學報
흑룡강팔일농은대학학보
JOURNAL OF HEILONGJIANG AUGUST FIRST LAND RECLAMATION UNIVERSITY
2015年
2期
19-22
,共4页
刘芳%郭晓红%廉华%马光恕%汪志强
劉芳%郭曉紅%廉華%馬光恕%汪誌彊
류방%곽효홍%렴화%마광서%왕지강
细叶百合%鳞茎%休眠%核酸%可溶性蛋白质
細葉百閤%鱗莖%休眠%覈痠%可溶性蛋白質
세협백합%린경%휴면%핵산%가용성단백질
Lilium pumilum%bulb%dormancy%nuclear acid%soluble protein
以细叶百合为试材,通过冷藏(5℃)解除鳞茎休眠,研究了细叶百合鳞茎解除休眠过程中核酸及可溶性蛋白质的变化规律。结果表明,冷藏过程中顶芽与鳞茎盘中核酸含量发生了明显的变化,顶芽中DNA、RNA含量及RNA/DNA比值在鳞茎冷藏36 d时变化最为明显,贮藏36 d是内外鳞片DNA及核酸总含量的转折点;顶芽和鳞茎盘冷藏0~36 d内可溶性蛋白质的含量升高,鳞茎不同部位可溶性蛋白质含量在冷藏48 d后迅速降低也是鳞茎开始解除休眠的重要体现。
以細葉百閤為試材,通過冷藏(5℃)解除鱗莖休眠,研究瞭細葉百閤鱗莖解除休眠過程中覈痠及可溶性蛋白質的變化規律。結果錶明,冷藏過程中頂芽與鱗莖盤中覈痠含量髮生瞭明顯的變化,頂芽中DNA、RNA含量及RNA/DNA比值在鱗莖冷藏36 d時變化最為明顯,貯藏36 d是內外鱗片DNA及覈痠總含量的轉摺點;頂芽和鱗莖盤冷藏0~36 d內可溶性蛋白質的含量升高,鱗莖不同部位可溶性蛋白質含量在冷藏48 d後迅速降低也是鱗莖開始解除休眠的重要體現。
이세협백합위시재,통과랭장(5℃)해제린경휴면,연구료세협백합린경해제휴면과정중핵산급가용성단백질적변화규률。결과표명,랭장과정중정아여린경반중핵산함량발생료명현적변화,정아중DNA、RNA함량급RNA/DNA비치재린경랭장36 d시변화최위명현,저장36 d시내외린편DNA급핵산총함량적전절점;정아화린경반랭장0~36 d내가용성단백질적함량승고,린경불동부위가용성단백질함량재랭장48 d후신속강저야시린경개시해제휴면적중요체현。
The changes of nuclear acid content and soluble protein content of Lilium pumilum in the process of bulb dormancy breaking at 5 ℃ were studied. Results showed that the changes of nucleic acid content in apical buds and basal plates of Lilium pumilum bulbs were obvious. The changes of DNA,RNA content and RNA/DNA ratio in apical buds were most obvious when bulb refrigerated 36 d,and 36 d was a turning point of the total amount of nucleic acid and DNA in inner and outer scales. The content of soluble protein in apical buds and basal plates increased within 0-36 d during refrigerated condition. The content of soluble protein in different parts of the bulbs reduced rapidly after 48 d,which was the important symbol of bulb for breaking dormancy.