中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2015年
2期
84-89
,共6页
霍奇金病%疱疹病毒4型,人%诊断,鉴别
霍奇金病%皰疹病毒4型,人%診斷,鑒彆
곽기금병%포진병독4형,인%진단,감별
Hodgkin Disease%Herpesvirus 4,Human%Diagnosis,differential
目的 分析北方地区霍奇金淋巴瘤(HL)的临床病理特征,与EB病毒(EBV)感染的相关性.同时对不同方式检测EBV感染的结果进行比较.方法 收集确诊为HL 235例患者临床病理资料,复阅HE及免疫组织化学染色切片以核实诊断和分型,并进行临床病理特征分析.全部病例采用免疫组织化学染色法检测LMP1蛋白,其中101例经原位杂交法检测EBV编码的RNA(EBER)明确肿瘤细胞EBV感染情况.结果 患者发病年龄以21～35岁最常见,其次为56～60岁.男性135例,女性100例.淋巴结内原发者217例,淋巴结外原发者8例.除3例为结节性淋巴细胞为主型HL(NLPHL)外,其余均为经典型HL.235例均进行了CD30、CD20、CD3检测:227例肿瘤细胞表达CD30(阳性率96.6％),53例肿瘤细胞不同程度表达CD20(阳性率22.5％),仅有1例肿瘤细胞表达CD3(阳性率0.4％).224例进行CD15染色,117例(52.2％)阳性;进行PAX-5检测的213例中有160例(75.1％)的肿瘤细胞呈弱到中等表达.235例肿瘤细胞LMP1的免疫组织化学染色有72例(30.6％)阳性.而其中101例同时进行了原位杂交检测的病例中有40例(39.6％) EBER检测阳性,其中30例表达LMP1,而EBER原位杂交检测阴性病例中仅5例表达LMP1,两种方法比较差异无统计学意义(P=0.3),总体符合率达85.2％.结论 北方地区HL发病年龄呈“双峰”特征,男性略多,发病部位以结内原发为主.病理类型最常见的是结节硬化型和混合细胞型.免疫组织化学染色法检测LMP1更为经济易行,且与EBER原位杂交具有高度一致性和高总体符合率.
目的 分析北方地區霍奇金淋巴瘤(HL)的臨床病理特徵,與EB病毒(EBV)感染的相關性.同時對不同方式檢測EBV感染的結果進行比較.方法 收集確診為HL 235例患者臨床病理資料,複閱HE及免疫組織化學染色切片以覈實診斷和分型,併進行臨床病理特徵分析.全部病例採用免疫組織化學染色法檢測LMP1蛋白,其中101例經原位雜交法檢測EBV編碼的RNA(EBER)明確腫瘤細胞EBV感染情況.結果 患者髮病年齡以21～35歲最常見,其次為56～60歲.男性135例,女性100例.淋巴結內原髮者217例,淋巴結外原髮者8例.除3例為結節性淋巴細胞為主型HL(NLPHL)外,其餘均為經典型HL.235例均進行瞭CD30、CD20、CD3檢測:227例腫瘤細胞錶達CD30(暘性率96.6％),53例腫瘤細胞不同程度錶達CD20(暘性率22.5％),僅有1例腫瘤細胞錶達CD3(暘性率0.4％).224例進行CD15染色,117例(52.2％)暘性;進行PAX-5檢測的213例中有160例(75.1％)的腫瘤細胞呈弱到中等錶達.235例腫瘤細胞LMP1的免疫組織化學染色有72例(30.6％)暘性.而其中101例同時進行瞭原位雜交檢測的病例中有40例(39.6％) EBER檢測暘性,其中30例錶達LMP1,而EBER原位雜交檢測陰性病例中僅5例錶達LMP1,兩種方法比較差異無統計學意義(P=0.3),總體符閤率達85.2％.結論 北方地區HL髮病年齡呈“雙峰”特徵,男性略多,髮病部位以結內原髮為主.病理類型最常見的是結節硬化型和混閤細胞型.免疫組織化學染色法檢測LMP1更為經濟易行,且與EBER原位雜交具有高度一緻性和高總體符閤率.
목적 분석북방지구곽기금림파류(HL)적림상병리특정,여EB병독(EBV)감염적상관성.동시대불동방식검측EBV감염적결과진행비교.방법 수집학진위HL 235례환자림상병리자료,복열HE급면역조직화학염색절편이핵실진단화분형,병진행림상병리특정분석.전부병례채용면역조직화학염색법검측LMP1단백,기중101례경원위잡교법검측EBV편마적RNA(EBER)명학종류세포EBV감염정황.결과 환자발병년령이21～35세최상견,기차위56～60세.남성135례,녀성100례.림파결내원발자217례,림파결외원발자8례.제3례위결절성림파세포위주형HL(NLPHL)외,기여균위경전형HL.235례균진행료CD30、CD20、CD3검측:227례종류세포표체CD30(양성솔96.6％),53례종류세포불동정도표체CD20(양성솔22.5％),부유1례종류세포표체CD3(양성솔0.4％).224례진행CD15염색,117례(52.2％)양성;진행PAX-5검측적213례중유160례(75.1％)적종류세포정약도중등표체.235례종류세포LMP1적면역조직화학염색유72례(30.6％)양성.이기중101례동시진행료원위잡교검측적병례중유40례(39.6％) EBER검측양성,기중30례표체LMP1,이EBER원위잡교검측음성병례중부5례표체LMP1,량충방법비교차이무통계학의의(P=0.3),총체부합솔체85.2％.결론 북방지구HL발병년령정“쌍봉”특정,남성략다,발병부위이결내원발위주.병리류형최상견적시결절경화형화혼합세포형.면역조직화학염색법검측LMP1경위경제역행,차여EBER원위잡교구유고도일치성화고총체부합솔.
Objective To study the clinicopathologic features of Hodgkin lymphoma (HL) occurring in northern China,association with Epstein-Barr virus (EBV) infection and concordance between EBV protein immunohistochemistry (IHC) and in-situ hybridization (ISH).Methods Two hundred and thirty-five cases were collected and their HE and IHC slides were reviewed to confirm the diagnosis and sort of HLs.All cases were performed with IHC staining for LMP-1 protein and ISH of EBV-encoded RNAs (EBER) was done in 101 cases to detect the existence of EBV.Results The incidence peak was between age 25 and 35 years,followed by another peak between age 56 to 60 years.There were 135 males and 100 females.The tumor involved lymph nodes in 217 cases,and extranodal sites in 18 cases.There were 3 cases of nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) and 232 cases of classical Hodgkin lymphoma.All tumors were stained for CD30,CD20,CD3.CD30 was expressed in 227 cases (96.6％),CD20 was expressed in 53 cases (22.5％) with different level of intensity.CD3 was expressed only in 1 case (0.4％).CD15 staining was performed in 224 cases and 117 (52.2％) cases were positive.PAX-5 were performed in 213 cases and 160 (75.1％) cases showed weak to moderate expressions.Two hundred and thirty-five cases were immunohistochemically stained with LMP1 and 72 (30.6％) cases were positive.Meanwhile,EBER ISH were applied in 101 cases,and 40 cases (39.6％) were found positive.LMP1 was expressed in 30 cases among those EBER-positive cases,while LMP1 was only detected in 5 cases of the EBER-negative cases.There was no statistically significantce between LMP1 IHC and EBER ISH by pared chi-square test (P =0.3),the overall concordance rate was 85.2％.Conclusions There was a bimodal age distribution in our group of HL cases from the northern part of China,with slight male predominance and mainly nodal involvement.Nodular sclerosis (NS) and mixed cellularity (MC) were major histologic subtypes.When it was compared with the EBER ISH method in detection EBV infection of HL,the more economical and convenient LMP1 IHC showed both high degree of consistency and overall concordance rate.
