中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2015年
2期
128-131
,共4页
董晨%张雪峰%鲍倡俊%朱叶飞%庄菱%谈忠鸣%钱慧敏%汤奋扬
董晨%張雪峰%鮑倡俊%硃葉飛%莊蔆%談忠鳴%錢慧敏%湯奮颺
동신%장설봉%포창준%주협비%장릉%담충명%전혜민%탕강양
弧菌,霍乱,01%基因,细菌%电泳,凝胶,脉冲场
弧菌,霍亂,01%基因,細菌%電泳,凝膠,脈遲場
호균,곽란,01%기인,세균%전영,응효,맥충장
Vibrio cholera O1%Genes,bacteria%Electrophoresis,gel,pulsed-field
目的 了解江苏省一起霍乱暴发疫情分离株的耐药性及病原学特征.方法 对2010年9月江苏省淮安市某中学发生的一起霍乱暴发疫情所分离的4株霍乱弧菌分离株进行药物敏感性实验、生物分型实验、9种毒力相关基因检测、ctxB基因序列分析及PFGE分子分型分析.结果 分离的4株霍乱弧菌对磺胺甲噁唑/甲氧苄啶、红霉素、链霉素耐药,对阿米卡星、诺氟沙星、环丙沙星、庆大霉素、氯霉素、氨苄青霉素敏感;生物分型实验结果显示,4株菌均为埃尔托型霍乱弧菌;均携带所测9种毒力相关基因,即ctxA、∞e、zot、toxR、tcpI、ompU、rtxC、tcpA、hlyA基因,且CTXB氨基酸序列为古典型;4株菌经Not Ⅰ酶切后的PFGE图谱为同一型别;经SfiⅠ酶切后的PFGE图谱分为2个型别.结论 此次暴发疫情的病原为产毒的非典型埃尔托霍乱弧菌流行株,且来自于共同的传染源,药物敏感实验可指导临床用药,以减少耐药株的产生.
目的 瞭解江囌省一起霍亂暴髮疫情分離株的耐藥性及病原學特徵.方法 對2010年9月江囌省淮安市某中學髮生的一起霍亂暴髮疫情所分離的4株霍亂弧菌分離株進行藥物敏感性實驗、生物分型實驗、9種毒力相關基因檢測、ctxB基因序列分析及PFGE分子分型分析.結果 分離的4株霍亂弧菌對磺胺甲噁唑/甲氧芐啶、紅黴素、鏈黴素耐藥,對阿米卡星、諾氟沙星、環丙沙星、慶大黴素、氯黴素、氨芐青黴素敏感;生物分型實驗結果顯示,4株菌均為埃爾託型霍亂弧菌;均攜帶所測9種毒力相關基因,即ctxA、∞e、zot、toxR、tcpI、ompU、rtxC、tcpA、hlyA基因,且CTXB氨基痠序列為古典型;4株菌經Not Ⅰ酶切後的PFGE圖譜為同一型彆;經SfiⅠ酶切後的PFGE圖譜分為2箇型彆.結論 此次暴髮疫情的病原為產毒的非典型埃爾託霍亂弧菌流行株,且來自于共同的傳染源,藥物敏感實驗可指導臨床用藥,以減少耐藥株的產生.
목적 료해강소성일기곽란폭발역정분리주적내약성급병원학특정.방법 대2010년9월강소성회안시모중학발생적일기곽란폭발역정소분리적4주곽란호균분리주진행약물민감성실험、생물분형실험、9충독력상관기인검측、ctxB기인서렬분석급PFGE분자분형분석.결과 분리적4주곽란호균대광알갑오서/갑양변정、홍매소、련매소내약,대아미잡성、낙불사성、배병사성、경대매소、록매소、안변청매소민감;생물분형실험결과현시,4주균균위애이탁형곽란호균;균휴대소측9충독력상관기인,즉ctxA、∞e、zot、toxR、tcpI、ompU、rtxC、tcpA、hlyA기인,차CTXB안기산서렬위고전형;4주균경Not Ⅰ매절후적PFGE도보위동일형별;경SfiⅠ매절후적PFGE도보분위2개형별.결론 차차폭발역정적병원위산독적비전형애이탁곽란호균류행주,차래자우공동적전염원,약물민감실험가지도림상용약,이감소내약주적산생.
Objective To assess the antibiotic resistance and molecular characterization of cholera strains and to provide basis for clinical treatment and prevention of cholera.Methods 4 stains isolated from an outbreak of cholera epidemic in Huai'an City in Jiangsu province in September 2010 were characterized using antibiotic susceptibility,biotype analysis,virluence genes detection,ctxB gene sequencing,and PFGE analysis.Results The 4 strains were all resistant to sulphamethoxazole/trimethoprim,erythromycin,streptomycin.High drug susceptibility of the samples was found to 6 kinds of antibiotics such as amikacin,norfloxacin,ciprofloxacin,gentamicin,chloramphenicol,ampicillin.The isolates expressed phenotypic traits of both serogroup O1 ogawa and El Tor and carried 9 kinds of virulence genes,ctxA,ace,zot,toxR,tcpI,ompU,rtxC,tcpA,and hlyA gene.They were also identified as harboring the classical ctxB genotype based on amino acid residue substitutions.The PFGE profiles of Not Ⅰ showed a single banding pattern,while Sfi Ⅰ's was 2 banding patterns.Conclusion The bacterium type of Vibrio cholerae causing the epidemic outbreak of cholera belonged to the atypical EL Tor variant which was also identified as toxicogenic strain.The mapping of the strains prompted that there should be the common contamination source.Drug sensitivity test can guide the clinical drug use,in order to reduce the emergence of resistant strains.
