中国麻风皮肤病杂志
中國痳風皮膚病雜誌
중국마풍피부병잡지
CHINA JOURNAL OF LEPROSY AND SKIN DISEASES
2015年
4期
195-197,208
,共4页
佘晓东%高盈%张莉莉%沈永年%Li Dongmei%刘维达
佘曉東%高盈%張莉莉%瀋永年%Li Dongmei%劉維達
사효동%고영%장리리%침영년%Li Dongmei%류유체
白念珠菌%线粒体%细胞壁%毒力
白唸珠菌%線粒體%細胞壁%毒力
백념주균%선립체%세포벽%독력
Candida albicans%mitochondrial%cell wall%virulence
目的：明确白念珠菌线粒体功能缺陷对菌株细胞壁结构及致病力的影响。方法：通过透射电镜观察野生株与缺陷株胞壁结构的差异；利用 qPCR检测野生株与缺陷株细胞壁成分 MNN4、PHR3,常见黏附因子ALS1、ALS3及毒力因子SAP9、SAP10的表达；利用小鼠系统感染模型明确缺陷株与野生株在小鼠致死率及脏器菌载量等方面的差异。结果：与野生株相比,透射电镜显示缺陷株细胞壁整体结构较为疏松,胞壁较薄,胞壁内外膜境界模糊；qPCR显示缺陷株细胞壁成分、黏附因子、毒力因子表达显著降低；动物模型显示缺陷株无法导致小鼠系统性感染死亡,肝、脾、肾脏器菌载量在接种后第3日明显降低。结论：白念珠菌线粒体功能缺陷会导致其胞壁完整性,并下调主要胞壁成分及毒力因子的表达,从而导致菌株致病力显著减弱。
目的：明確白唸珠菌線粒體功能缺陷對菌株細胞壁結構及緻病力的影響。方法：通過透射電鏡觀察野生株與缺陷株胞壁結構的差異；利用 qPCR檢測野生株與缺陷株細胞壁成分 MNN4、PHR3,常見黏附因子ALS1、ALS3及毒力因子SAP9、SAP10的錶達；利用小鼠繫統感染模型明確缺陷株與野生株在小鼠緻死率及髒器菌載量等方麵的差異。結果：與野生株相比,透射電鏡顯示缺陷株細胞壁整體結構較為疏鬆,胞壁較薄,胞壁內外膜境界模糊；qPCR顯示缺陷株細胞壁成分、黏附因子、毒力因子錶達顯著降低；動物模型顯示缺陷株無法導緻小鼠繫統性感染死亡,肝、脾、腎髒器菌載量在接種後第3日明顯降低。結論：白唸珠菌線粒體功能缺陷會導緻其胞壁完整性,併下調主要胞壁成分及毒力因子的錶達,從而導緻菌株緻病力顯著減弱。
목적：명학백념주균선립체공능결함대균주세포벽결구급치병력적영향。방법：통과투사전경관찰야생주여결함주포벽결구적차이；이용 qPCR검측야생주여결함주세포벽성분 MNN4、PHR3,상견점부인자ALS1、ALS3급독력인자SAP9、SAP10적표체；이용소서계통감염모형명학결함주여야생주재소서치사솔급장기균재량등방면적차이。결과：여야생주상비,투사전경현시결함주세포벽정체결구교위소송,포벽교박,포벽내외막경계모호；qPCR현시결함주세포벽성분、점부인자、독력인자표체현저강저；동물모형현시결함주무법도치소서계통성감염사망,간、비、신장기균재량재접충후제3일명현강저。결론：백념주균선립체공능결함회도치기포벽완정성,병하조주요포벽성분급독력인자적표체,종이도치균주치병력현저감약。
Objective:To determine the effects of Candida albicans mitochondrial dysfunction on cell wall structure, virulence and pathogenicity. Methods:The differences of cell wall structure between the wild-type strain and the deficient strain were observed under transmission electron microscopy. The expression of cell wall components MNN4, PHR3, adhesion factors ALS1, ALS3 and virulence factors SAP9, SAP10 in the wild-type and deficient strain were detected using qPCR. The differences of the fatality rate and organ CFU load between the wild-type and deficient strain were detected in mice system infection model. Results:Com-pared with the wild-type strain, the deficient strain showed that the cell wall was thinner, the structure was looser and the realm of inner and outer membrane of cell wall was fuzzier under transmission scanning electron microscope. The expression of cell wall components, adhesion factors and virulence factors reduced significant-ly and there was no mice death in the deficient strain group. The CFU in liver, spleen and kidney of the mice treated with the deficient strain after 3 days reduced significantly. Conclusion: Mitochondrial dysfunction in candida albicans damage the cell wall integrity, reduce the expression of the major cell wall components and virulence factors, resulting in reduction of the pathogenicity of strain.
