CAJ | 학술논문

目的：在大鼠脊髓不完全损伤后，观察蒙药珍宝丸对神经的再生修复作用及其对RhoA表达的调节作用。方法利用Allen’s改良方法建立脊髓不完全损伤的动物模型，把48只成年Wistar大鼠随机分为损伤组（A组，n＝24）和损伤后珍宝丸治疗组（B组，n＝24），B组在损伤脊髓后以珍宝丸灌胃治疗。A、B组按损伤后3、7、14 d分为3个时间点，每个时间点8只。分别在术后3、7、14 d对A、B组大鼠后肢运动功能进行BBB评分，再在各时间点处死动物，取受损脊髓行 H ．E染色及免疫组化染色观察形态学变化和RhoA在脊髓组织中的分布特点，同时对各组相应时间点行阳性细胞计数，并作比较。结果在7 d和14 d这两个时间点，B组的BBB评分明显高于A组，差异有统计学意义（ P＜0．05）。B组大鼠受损脊髓的病理形态改善。B组的RhoA蛋白表达量显著低于A组，差异有显著性（ P＜0．05）。结论大鼠急性脊髓损伤后RhoA表达显著升高，早期应用珍宝丸对RhoA的表达具有一定的抑制作用。
목적：재대서척수불완전손상후，관찰몽약진보환대신경적재생수복작용급기대RhoA표체적조절작용。방법이용Allen’s개량방법건립척수불완전손상적동물모형，파48지성년Wistar대서수궤분위손상조（A조，n＝24）화손상후진보환치료조（B조，n＝24），B조재손상척수후이진보환관위치료。A、B조안손상후3、7、14 d분위3개시간점，매개시간점8지。분별재술후3、7、14 d대A、B조대서후지운동공능진행BBB평분，재재각시간점처사동물，취수손척수행 H ．E염색급면역조화염색관찰형태학변화화RhoA재척수조직중적분포특점，동시대각조상응시간점행양성세포계수，병작비교。결과재7 d화14 d저량개시간점，B조적BBB평분명현고우A조，차이유통계학의의（ P＜0．05）。B조대서수손척수적병리형태개선。B조적RhoA단백표체량현저저우A조，차이유현저성（ P＜0．05）。결론대서급성척수손상후RhoA표체현저승고，조기응용진보환대RhoA적표체구유일정적억제작용。
Objective To investigate the effects of mongolian medicine Zhenbao pills on expression of RhoA in rats with acute incomplete spinal cord injury .Method Rat model of spinal cord injury was established by modified Allen’s method .Forty -eight adult Wistar rats were divided into the spinal cord injury group (group A ,n=2 4 ) and group B( n=2 4 ) treated with Zhenbao pills at random after spinal cord injury .The rats in group B was treated with Zhenbao pills (1 g/kg) once a day by gavage .At 3 d ,7 d and 1 4 d after operation , motor functions of the rat hind limbs were determined by the BBB (Basso Beattie and Bresnahan) ,and the rats in each group were killed at 3 d ,7 d and 1 4 d post operation for histopathology examination .Morphologic changes of spinal neurons were evaluated by HE staining and immunohistochemistry ,respectively .Results It was ob-served that at 7 d and 1 4 d after operation ,the BBC score of group B was significantly higher ( P<0 .0 5 ) than that of group A .However ,the expression levels of RhoA in group A were significantly higher ( P<0 .0 5 ) than that in group B .Conclusion The expression of RhoA was markedly increased in rats with acute spinal cord in-jury ,and early use of Zhenbao pills may inhibit the expression of RhoA .