CAJ | 학술논문

目的：探讨低温条件保存血小板的最佳方法。方法随机选取10人份血小板，在5％二甲基亚砜（DM SO ）的条件下分别放入低温冰箱（－80℃）及液氮中冷冻保存。分别检测经两种方式保存2月后的血小板计数（PL T ）、血小板平均体积（M PV ）、血小板体积分布宽度（PDW ）、PH值、乳酸脱氢酶（LDH ）浓度、磷脂酰丝氨酸（PS ）阳性率及CD62 p阳性率。结果两组低温保存复温后的血小板与新鲜血小板相比PH值无显著差异（ P＞0．05），PL T、M PV、PDW、LDH、PS阳性率及CD62 p阳性率均有显著差异（ P＜0．05）。两组低温保存方式间各项指标比较均无显著差异（P＞0．05）。结论液氮及低温冰箱保存均会在一定程度上损伤血小板，两种保存方式比较无明显区别。
목적：탐토저온조건보존혈소판적최가방법。방법수궤선취10인빈혈소판，재5％이갑기아풍（DM SO ）적조건하분별방입저온빙상（－80℃）급액담중냉동보존。분별검측경량충방식보존2월후적혈소판계수（PL T ）、혈소판평균체적（M PV ）、혈소판체적분포관도（PDW ）、PH치、유산탈경매（LDH ）농도、린지선사안산（PS ）양성솔급CD62 p양성솔。결과량조저온보존복온후적혈소판여신선혈소판상비PH치무현저차이（ P＞0．05），PL T、M PV、PDW、LDH、PS양성솔급CD62 p양성솔균유현저차이（ P＜0．05）。량조저온보존방식간각항지표비교균무현저차이（P＞0．05）。결론액담급저온빙상보존균회재일정정도상손상혈소판，량충보존방식비교무명현구별。
Objective To explore the best way of platelet cryopreservation .Methods Selected 1 0 fresh platelet -rich plasmas (FPRPs) with 5% DMSO were cryopreserved in the -8 0 ℃ hypothermia refrigerator and liquid nitrogen for 2 months .The aliquots were thawed by 3 7 ℃ water bath for analysis .The markers observed included platelet account ,mean platelet volume ,platelet distribute width ,pH ,LDH ,phosphoryl serine express and CD6 2 p express .Results All the indexes between the two groups of cryopreservation method had no signifi-cant difference( P>0 .0 5 ) .Compared with fresh platelets ,PH had no significant difference in two groups of cryopreservated platelets(P>0 .05 ) .PLT ,MPV ,PDW ,LDH ,PS express and CD62p express had significant difference( P<0 .0 5 ) .Conclusion To some extent ,both the -8 0 ℃ hypothermia refrigerator and liquid nitro-gen cryopreservation were damaged platelet .There are no obvious difference between the two kinds of cryop-reservation methods .