CAJ | 학술논문

目的：构建pcDNATM6.2-GW/EmGFP-miR-214表达载体,并检测其对肝癌Hep3B细胞增殖的影响。方法以人的基因组DNA为模板,利用PCR扩增miR-214前体序列,构建pcDNATM6.2-GW/EmGFP-miR-214表达载体,序列比对及实时定量PCR验证miR-214表达载体是否构建成功,MTT实验检测miR-214对肝癌Hep3B细胞增殖的影响。结果序列比对及实时定量PCR验证miR-214表达载体构建成功。将成功构建的miR-214表达载体导入肝癌细胞Hep3B,发现其对细胞增殖具有抑制作用。结论本研究成功构建miR-214表达载体并证明miR-214可能参与肝癌发展的过程。
목적：구건pcDNATM6.2-GW/EmGFP-miR-214표체재체,병검측기대간암Hep3B세포증식적영향。방법이인적기인조DNA위모판,이용PCR확증miR-214전체서렬,구건pcDNATM6.2-GW/EmGFP-miR-214표체재체,서렬비대급실시정량PCR험증miR-214표체재체시부구건성공,MTT실험검측miR-214대간암Hep3B세포증식적영향。결과서렬비대급실시정량PCR험증miR-214표체재체구건성공。장성공구건적miR-214표체재체도입간암세포Hep3B,발현기대세포증식구유억제작용。결론본연구성공구건miR-214표체재체병증명miR-214가능삼여간암발전적과정。
Objective To construct the miR-214 expression vector and explore its effect on the proliferation of hepatocellular carcino-ma cell Hep3B. Methods The precursor of human miR-214 gene was amplified by polymerase chain reaction(PCR)from the human genomic DNA. The miR-214 expression vector pcDNATM6. 2-GW/EmGFP-miR-214 was constructed and then verified by sequence a-lignment and real time PCR. Results The pcDNATM6. 2-GW/EmGFP-miR-214 expression vector was successfully constructed and confirmed by the results of sequence alignment and real time PCR. MTT assay showed that miR-214 inhibited the proliferation of Hep3B cells. Conclusion The miR-214 might be involved in the development of hepatocellular carcinoma.