CAJ | 학술논문

目的：研究去乙酰化酶6(Sirtuin 6,SIRT6)在胃癌组织中的表达及其重要意义。方法应用Western blot免疫印迹法检测25例胃癌组织及癌旁正常组织中SIRT6的表达水平；选择Western blot中癌旁组织与癌组织中SIRT6差异有统计学意义的17例患者中的胃癌组织及对应癌旁正常黏膜组织,提取相应组织中的总RNA,逆转录成cDNA,采用实时荧光定量PCR ( qRT-PCR)方法检测SIRT6 mRNA的表达情况。结果25例中有17例患者的SIRT6在胃癌组织中的表达水平低于癌旁正常组织；qRT-PCR方法检测胃癌组织中SIRT6的mRNA表达水平显著低于癌旁正常黏膜组织,差异有统计学意义( P<0.01)。
목적：연구거을선화매6(Sirtuin 6,SIRT6)재위암조직중적표체급기중요의의。방법응용Western blot면역인적법검측25례위암조직급암방정상조직중SIRT6적표체수평；선택Western blot중암방조직여암조직중SIRT6차이유통계학의의적17례환자중적위암조직급대응암방정상점막조직,제취상응조직중적총RNA,역전록성cDNA,채용실시형광정량PCR ( qRT-PCR)방법검측SIRT6 mRNA적표체정황。결과25례중유17례환자적SIRT6재위암조직중적표체수평저우암방정상조직；qRT-PCR방법검측위암조직중SIRT6적mRNA표체수평현저저우암방정상점막조직,차이유통계학의의( P<0.01)。
Objective To investigate the expression and its significance of SIRT6 in gastric carcinoma. Methods The expression levels of SIRT6 protein in gastric carcinoma tissues and normal tissue adjacent to cancer from 25 cases were detected by Western blot. The total RNA was extracted from gastric cancer tissues and their corresponding non-tumor normal tissues in 17 cases of abnormal SIRT6 expression, and then cDNA was synthesized. SIRT6 mRNA level was detected using real-time fluorescence quantitative PCR method. Results The protein expression of SIRT6 was lower in gastric cancer tissues than that in normal tissue adjacent to cancer in 17 of 25 cases. The mRNA level of SIRT6 in gastric cancer tissue was significantly lower in gastric cancer tissues than that of the corre-sponding non-tumor normal tissues(P<0. 01). Conclusion The low expression of SIRT6 protein and mRNA in gastric cancer sug-gests that SIRT6 expression might be associated with the development of gastric cancer.