CAJ | 학술논문

目的：探索β1-肾上腺素能受体(ADRB1)Arg389>Gly多态性与收缩性心力衰竭(SHF)患病风险是否独立相关。方法采用回顾性病例对照研究,获得1716名受试者的基因血测序数据( sanger method),同时取得相关临床实验室检测、心电图和超声心动图检查的结果,并根据左室射血分数( LVEF)小于50%或二维超声心动图多节段的室壁收缩运动减低分为SHF组和无SHF组,观察ADRB1 Arg389Gly多态性、NT-proBNP、LVEF等指标。结果在无SHF人群中,ADRB1 Arg389和Gly389等位基因频数(频率)分别为2127(0.715)和849(0.285)(χ2=0.272,P>0.05),SHF人群的Arg389和Gly389的等位基因频数(频率)分别为331(0.726)和125(0.274)(χ2=1.892, P >0.05)。简单相关分析表明 ADRB1基因多态性(Arg389Arg,Arg389Gly和Gly389Gly)与LVEF、NT-proBNP和SHF之间无显著性相关(r分别为-0.004、0.007和0.007,P均>0.05)。进一步通过Logistic回归模型,调整心力衰竭的相关危险因素后,与基线Arg389Arg相比,ADRB1突变体与SHF患病风险无显著性独立相关(P均>0.05)。结论 ADRB1 Arg389Gly多态性与SHF患病风险无独立的相关性。
목적：탐색β1-신상선소능수체(ADRB1)Arg389>Gly다태성여수축성심력쇠갈(SHF)환병풍험시부독립상관。방법채용회고성병례대조연구,획득1716명수시자적기인혈측서수거( sanger method),동시취득상관림상실험실검측、심전도화초성심동도검사적결과,병근거좌실사혈분수( LVEF)소우50%혹이유초성심동도다절단적실벽수축운동감저분위SHF조화무SHF조,관찰ADRB1 Arg389Gly다태성、NT-proBNP、LVEF등지표。결과재무SHF인군중,ADRB1 Arg389화Gly389등위기인빈수(빈솔)분별위2127(0.715)화849(0.285)(χ2=0.272,P>0.05),SHF인군적Arg389화Gly389적등위기인빈수(빈솔)분별위331(0.726)화125(0.274)(χ2=1.892, P >0.05)。간단상관분석표명 ADRB1기인다태성(Arg389Arg,Arg389Gly화Gly389Gly)여LVEF、NT-proBNP화SHF지간무현저성상관(r분별위-0.004、0.007화0.007,P균>0.05)。진일보통과Logistic회귀모형,조정심력쇠갈적상관위험인소후,여기선Arg389Arg상비,ADRB1돌변체여SHF환병풍험무현저성독립상관(P균>0.05)。결론 ADRB1 Arg389Gly다태성여SHF환병풍험무독립적상관성。
Objective To explore the association between β1-adrenergic receptor ( ADRB1 ) Arg389>Gly polymorphisms and systolic heart failure(SHF). Methods In this retrospective case-control study,the gene sequencing data of ADRB1(Sanger method) were obtained from 1 716 subjects. Blood sample collection, clinical laboratory measurement, electrocardiogram and echocardiography were performed. According to the left ventricular ejection fraction( LVEF) of ventricular wall contraction less than 50% or multi segmental re-duction in two-dimensional echocardiography, the subjects were divided into SHF group and non-SHF group, and the ADRB1 Arg389Gly polymorphism,NT-proBNP,LVEF were observed. Results In non-SHF subjects, allele frequencies(rate) of ADRB1 Arg389 and Gly389 were 2 127(0. 715) and 849(0. 285) (χ2 =0. 272, P=0. 602). In SHF patients, allele frequencies(rate) of Arg389 and Gly389 were 331(0. 726) and 125(0. 274) (χ2 =1. 892,P=0. 169). Bivariate correlation analysis indicated that ADRB1 polymorphisms(Arg389Arg, Arg389Gly and Gly389Gly) were not correlated with LVEF, NT-proBNP and SHF (r= -0. 004,P=0. 872;r=0. 007,P=0. 791 and r=0. 007,P =0. 758 respectively). Logistic regression analysis showed that after adjusting heart failure relevant risk factors,compared with Arg389Arg,ADRB1 polymorphism was not independently associated with the risk of SHF(P>0. 05). Conclusion ADRB1 Arg389Gly polymorphisms are not independently associated with the risk of SHF.