CAJ | 학술논문

目的：探讨斯氏狸殖吸虫病敏感性和特异性较好的免疫学诊断技术。方法采用金标渗滤法( DIGFA-kit)检测秦巴山区十堰市居民、实验大鼠和对照组感染旋毛虫大鼠、感染血吸虫兔、粪检确诊蛔虫病人和正常健康大鼠血清IgG抗体。结果 DIGFA-kit检测十堰市居民和实验大鼠血清斯氏狸殖吸虫IgG抗体的检出率分别为4.9%和97.6%。从第2周开始，实验大鼠即能测出抗体，第4-9周达高峰。对照组除1份血吸虫兔血清阳性外均为阴性。结论金标渗滤试法对斯氏狸殖吸虫病血清IgG抗体的检测有较好的敏感性和特异性。
목적：탐토사씨리식흡충병민감성화특이성교호적면역학진단기술。방법채용금표삼려법( DIGFA-kit)검측진파산구십언시거민、실험대서화대조조감염선모충대서、감염혈흡충토、분검학진회충병인화정상건강대서혈청IgG항체。결과 DIGFA-kit검측십언시거민화실험대서혈청사씨리식흡충IgG항체적검출솔분별위4.9%화97.6%。종제2주개시，실험대서즉능측출항체，제4-9주체고봉。대조조제1빈혈흡충토혈청양성외균위음성。결론금표삼려시법대사씨리식흡충병혈청IgG항체적검측유교호적민감성화특이성。
Objective To explore a better immunologic diagnosis technology in detection of the Paragonimiasis skrjabini. Methods The DIGFA was used to test the anti-Paragonimiasis skrjabini antibody in sera from the crowd in popular area, experimental rats, and the rats infected with trichina, the rabbits infected with schistosoma japonicum, patients with ascaris, healthy rats in control group. Results The positive rats of the antibody in sera from crowd in popular area and infected rats all were 4. 9% and 97. 6%, respectively. The antibody was found at 2 week after infection in experimental rats, and the positive rate reached 90% at 4 week and lasted until week 9. The serum was negative for Paragonimiasis skrjabini in control group except one rabbit. Conclusion DIGFA has a high sen-sitivity and specificity for early diagnosis of paragonimiasis.