CAJ | 학술논문

目的：：明确丹参酮联合氧化苦参碱对银屑病模型细胞增殖、颗粒层形成及小鼠阴道上皮有丝分裂的影响。方法：体外培养HaCaT细胞、构建小鼠尾鳞片形成模型和小鼠阴道上皮细胞过度增殖模型，构建成功后分别分组给予丹参酮、氧化苦参碱及丹参酮与氧化苦参碱联合的处理。结果：丹参酮，氧化苦参碱及联合用药对HaCaT细胞的增殖均具有浓度依赖性抑制作用且丹参酮和氧化苦参碱对HaCaT细胞增殖的抑制作用具有协同效应；丹参酮、氧化苦参碱及联合用药均可促进小鼠尾部皮肤颗粒层的形成，可抑制小鼠阴道上皮有丝分裂，联合用药组作用最强。结论：丹参酮和氧化苦参碱联合应用具有抑制HaCaT细胞增殖和阴道上皮细胞分裂，促进颗粒层形成的作用。
목적：：명학단삼동연합양화고삼감대은설병모형세포증식、과립층형성급소서음도상피유사분렬적영향。방법：체외배양HaCaT세포、구건소서미린편형성모형화소서음도상피세포과도증식모형，구건성공후분별분조급여단삼동、양화고삼감급단삼동여양화고삼감연합적처리。결과：단삼동，양화고삼감급연합용약대HaCaT세포적증식균구유농도의뢰성억제작용차단삼동화양화고삼감대HaCaT세포증식적억제작용구유협동효응；단삼동、양화고삼감급연합용약균가촉진소서미부피부과립층적형성，가억제소서음도상피유사분렬，연합용약조작용최강。결론：단삼동화양화고삼감연합응용구유억제HaCaT세포증식화음도상피세포분렬，촉진과립층형성적작용。
Objective:To determine the effects of Tanshinone combined with Oxymatrine on the prolifera-tion of HaCaT cells, stratum granulosum formation and mitosis of mouse vaginal epithelia. Methods: HaCaT cells were cultured in vitro and the tail scale formation mouse model and vaginal epithelial cells excessive pro-liferation mouse model were constructed. The HaCaT cells and the mice in the two models were treated with Tanshinone, Oxymatrine and Tanshinone combined with Oxymatrine. Results: The proliferation of HaCaT cells was inhibited after treated with Tanshinone, Oxymatrine and Tanshinone combined with Oxymatrine in a concentration-dependent manner. Oxymatrine and Tanshinone cooperated mutually in the process of inhibiting the proliferation of HaCaT cells. The granular cells in the flake of the mouse’ s tail were increased and the mi-tosis of mouse vaginal epithelia was inhibited after treated with Tanshinone, Oxymatrine and Tanshinone com-bined with Oxymatrine. Conclusion:Tanshinone combined with Oxymatrine inhibite the HaCaT cells prolifera-tion and mitosis of mouse vaginal epithelia and promote the formation of stratum granulosum.