CAJ | 학술논문

目的：探讨抗感染对伴炎症兔颊VX2鳞状细胞癌（鳞癌）组织中树突状细胞（DCs）表面分子HLA-DR和CD86表达的影响。方法通过接种VX2瘤粒、形成机械创伤、饮用高糖黏性牛奶的方式，建立兔颊VX2鳞癌炎症模型。实验分为4组。A组（12只）：颊癌伴炎症模型兔，连续3?d普鲁卡因青霉素肌注和替硝唑片灌胃。B组（12只）：颊癌伴炎症模型兔，连续3?d生理盐水肌注和阿司匹林灌胃。C组（12只）：颊癌伴炎症模型兔，伴有局部炎症的兔颊癌，连续3?d生理盐水肌注和灌胃。D组（10只）：单纯颊癌模型兔，连续3?d生理盐水灌胃和肌注。实验结束后处死所有兔，收集肿瘤标本，流式细胞仪检测肿瘤组织中DCs表面分子HLA-DR和CD86的表达。结果 HLA-DR单独表达率、HLA-DR和CD86同时表达率均为A组>D组>B组>C组，CD86单独表达率为A组>D组>B组及C组（P<0.05）。结论抗感染治疗能明显提高伴炎症的兔颊癌组织中DCs表面分子HLA-DR和CD86的表达。
목적：탐토항감염대반염증토협VX2린상세포암（린암）조직중수돌상세포（DCs）표면분자HLA-DR화CD86표체적영향。방법통과접충VX2류립、형성궤계창상、음용고당점성우내적방식，건립토협VX2린암염증모형。실험분위4조。A조（12지）：협암반염증모형토，련속3?d보로잡인청매소기주화체초서편관위。B조（12지）：협암반염증모형토，련속3?d생리염수기주화아사필림관위。C조（12지）：협암반염증모형토，반유국부염증적토협암，련속3?d생리염수기주화관위。D조（10지）：단순협암모형토，련속3?d생리염수관위화기주。실험결속후처사소유토，수집종류표본，류식세포의검측종류조직중DCs표면분자HLA-DR화CD86적표체。결과 HLA-DR단독표체솔、HLA-DR화CD86동시표체솔균위A조>D조>B조>C조，CD86단독표체솔위A조>D조>B조급C조（P<0.05）。결론항감염치료능명현제고반염증적토협암조직중DCs표면분자HLA-DR화CD86적표체。
ObjectiveTo?investigate?the?effects?of?anti-infection?treatment?on?the?expressions?of?antigen-presenting-related?membrane-surface?molecules?HLA-DR?and?CD86?in?dendritic?cells?(DCs)?in?rabbit?buccal?VX2?squamous?cell?carcinoma?tissue?complicated?with?local?inflammation.?Methods???Rabbit?buccal?VX2?squamous?cell?carcinoma?with?local?inflammation?models?that?were?established?by?inflammation?was?induced?by?inoculation?VX2?tumor,?mechanical?trauma,?and?drinking?of?milk?with?high?sugar?viscosity.?The?animals?were?divided?into?four?groups.?Group?A?(n=12):?rabbit?buccal?VX2?squamous?cell?carcinoma?with?local?inflammation,?procaine?penicillin?was?intramuscularly?given,?and?tinidazole?tablets?were?given?by?gavage?for?three?consecutive?days.?Group?B?(n=12): rabbit buccal VX2 squamous cell carcinoma with local inflammation, normal?saline?was?intramuscularly?given,?and?aspirin?were?given?by?gavage?for?three?consecutive?days.?Group?C?(n=12): rabbit buccal?VX2?squamous?cell?carcinoma?with?local?inflammation,?normal?saline?was?given?intramuscularly?and?by?gavage?for?three?consecutive?days.?Group?D?(n=10): rabbit buccal VX2 squamous cell carcinoma, normal saline was given intramuscu-larly?and?by?gavage?for?three?consecutive?days.?All?the?rabbits?were?sacrificed?for?collection?of?tumor?specimens,?and?the?expression?levels?of?membrane-surface?HLA-DR?and?CD86?in?DCs?of?tumor?specimens?were?detected?viaflow?cytometry.?Results???The?positive?expression?rate?of?HLA-DR?and?the?double?positive?expression?rate?of?HLA-DR?and?CD86?were?group?A>?group?D>?group?B>?group?C.?The?positive?expression?rate?of?CD86?were?group?A>?group?D>?group?B?and?group?C?(P<0.05).?Conclusion???Anti-infection?treatment?significantly?increased?the?expressions?of?HLA-DR?and?CD86?in?DCs?of?rabbit?buccal?VX2?squamous?cell?carcinoma?tissue?complicated?with?local?inflammation.