CAJ | 학술논문

目的：研究纤维蛋白胶与人牙囊细胞（DFCs）二维（2D）及三维（3D）结合模式对细胞存活、增殖及凋亡的影响，评估纤维蛋白胶作为细胞移植载体的可行性及有效性。方法采用细胞膜绿色荧光探针（DIO）对存活DFCs细胞膜进行标记，采用ImageJ2x软件细胞计数；采用碘化丙啶（PI）对凋亡细胞进行染色。结果纤维蛋白胶在3D-2和2D-1复合模式下的降解速度快于3D-1模式；纤维蛋白原浓度为15?mg·mL-1辅以凝血酶浓度2?U·mL-1条件下， DFCs能够存活并生长良好，尤其在3D-1结合模式下更有利于细胞增殖及伸展。结论纤维蛋白胶可作为一种有效的细胞移植载体，纤维蛋白胶不同的结合方式对细胞的增殖活性有一定的影响，3D-1复合模式更有利于细胞的存活增殖。
목적：연구섬유단백효여인아낭세포（DFCs）이유（2D）급삼유（3D）결합모식대세포존활、증식급조망적영향，평고섬유단백효작위세포이식재체적가행성급유효성。방법채용세포막록색형광탐침（DIO）대존활DFCs세포막진행표기，채용ImageJ2x연건세포계수；채용전화병정（PI）대조망세포진행염색。결과섬유단백효재3D-2화2D-1복합모식하적강해속도쾌우3D-1모식；섬유단백원농도위15?mg·mL-1보이응혈매농도2?U·mL-1조건하， DFCs능구존활병생장량호，우기재3D-1결합모식하경유리우세포증식급신전。결론섬유단백효가작위일충유효적세포이식재체，섬유단백효불동적결합방식대세포적증식활성유일정적영향，3D-1복합모식경유리우세포적존활증식。
ObjectiveThis?study?explores?the?effects?of?different?fibrin?glue?combination?modes?on?the?survival,?prolife-ration,?and?apoptosis?of?dental?follicle?cells?(DFCs),?as?well?as?to?evaluate?the?feasibility?and??effectiveness?of?fibrin?glue?as?trans-plantation?material.?Methods???The?membranes?of?surviving?DFCs?were?marked?using?3,3’-dioctadecyloxa?carbocyanine?per-chlorate?(DIO),?and?the?cell?number?was?counted?by?using?ImageJ2x?software.?The?apoptotic?cells?were?marked?with?prodium?iodide?(PI).?Results???Compared?with?that?of?the?3D-2?and?2D-1?groups,?the?degradation?speed?of?the?3D-1?group?was?the?slowest.?DFCs?could?survive?and?grow?well?in?fibrinogen?with?a?concentration?of?15?mg·mL-1?supplemented?with?thrombin?with?a?concentration?of?2?U·mL-1.?In?particular,?the?3D-1?combination?mode?was?significantly?conducive?to?cell?proliferation?and?stretching.?Conclusion???Fibrin?glue?can?be?used?as?an?effective?cell?transplantation?material.?The?different?combination?modes?have?certain?effects?on?cell?proliferation.?The?3D-1?combination?mode?is?more?conducive?to?the?survival?and?prolifera-tion?of?DFCs?than?other?modes.