中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2015年
1期
48-51
,共4页
电刺激疗法%脑%再灌注损伤%四肢%缺血后处理
電刺激療法%腦%再灌註損傷%四肢%缺血後處理
전자격요법%뇌%재관주손상%사지%결혈후처리
Electric stimulation therapy%Brain%Reperfusion injury%Extremities%Ischemic postconditioning
目的 评价肢体远隔缺血后处理联合电针后处理对大鼠局灶性脑缺血再灌注损伤的影响.方法 雄性SD大鼠70只,6周龄,体重220～ 250 g,采用随机数字表法分为5组(n=14):假手术组(S组)、缺血再灌注组(I/R组)、肢体远隔缺血后处理组(RLIP组)、电针后处理组(EAP组)和肢体远隔缺血后处理联合电针后处理组(LRIP+EAP组).采用大脑中动脉线栓阻塞120 min时再灌注的方法制备局灶性脑缺血再灌注损伤模型.于再灌注即刻实施肢体远隔缺血后处理,对双侧股动脉实施3次15 min阻断15 min开放;于再灌注即刻实施电针后处理,电针刺激大椎穴和百会穴,选用疏密波,频率15 Hz/2 Hz,强度1 mA,时间30 min.再灌注24 h时进行神经功能缺陷(NDS)评分,然后处死,取脑组织,测定脑梗死体积百分比,Nissil染色,光镜下观察海马CA1区病理学结果,进行存活神经元计数,并测定凋亡指数(AI).结果 与S组比较,其余4组NDS评分、脑梗死体积百分比、AI增加,存活神经元计数降低(P＜0.05);与I/R组比较,RLIP组、EAP组和RLIP+EAP组NDS评分、脑梗死体积百分比和AI降低,存活神经元计数升高(P＜0.05);与RLIP组和EAP组比较,RLIP+EAP组NDS评分、脑梗死体积百分比和AI降低,存活神经元计数升高(P＜0.05).结论 肢体远隔缺血后处理联合电针后处理减轻大鼠局灶性脑缺血再灌注损伤的效果优于单独一种方法.
目的 評價肢體遠隔缺血後處理聯閤電針後處理對大鼠跼竈性腦缺血再灌註損傷的影響.方法 雄性SD大鼠70隻,6週齡,體重220～ 250 g,採用隨機數字錶法分為5組(n=14):假手術組(S組)、缺血再灌註組(I/R組)、肢體遠隔缺血後處理組(RLIP組)、電針後處理組(EAP組)和肢體遠隔缺血後處理聯閤電針後處理組(LRIP+EAP組).採用大腦中動脈線栓阻塞120 min時再灌註的方法製備跼竈性腦缺血再灌註損傷模型.于再灌註即刻實施肢體遠隔缺血後處理,對雙側股動脈實施3次15 min阻斷15 min開放;于再灌註即刻實施電針後處理,電針刺激大椎穴和百會穴,選用疏密波,頻率15 Hz/2 Hz,彊度1 mA,時間30 min.再灌註24 h時進行神經功能缺陷(NDS)評分,然後處死,取腦組織,測定腦梗死體積百分比,Nissil染色,光鏡下觀察海馬CA1區病理學結果,進行存活神經元計數,併測定凋亡指數(AI).結果 與S組比較,其餘4組NDS評分、腦梗死體積百分比、AI增加,存活神經元計數降低(P＜0.05);與I/R組比較,RLIP組、EAP組和RLIP+EAP組NDS評分、腦梗死體積百分比和AI降低,存活神經元計數升高(P＜0.05);與RLIP組和EAP組比較,RLIP+EAP組NDS評分、腦梗死體積百分比和AI降低,存活神經元計數升高(P＜0.05).結論 肢體遠隔缺血後處理聯閤電針後處理減輕大鼠跼竈性腦缺血再灌註損傷的效果優于單獨一種方法.
목적 평개지체원격결혈후처리연합전침후처리대대서국조성뇌결혈재관주손상적영향.방법 웅성SD대서70지,6주령,체중220～ 250 g,채용수궤수자표법분위5조(n=14):가수술조(S조)、결혈재관주조(I/R조)、지체원격결혈후처리조(RLIP조)、전침후처리조(EAP조)화지체원격결혈후처리연합전침후처리조(LRIP+EAP조).채용대뇌중동맥선전조새120 min시재관주적방법제비국조성뇌결혈재관주손상모형.우재관주즉각실시지체원격결혈후처리,대쌍측고동맥실시3차15 min조단15 min개방;우재관주즉각실시전침후처리,전침자격대추혈화백회혈,선용소밀파,빈솔15 Hz/2 Hz,강도1 mA,시간30 min.재관주24 h시진행신경공능결함(NDS)평분,연후처사,취뇌조직,측정뇌경사체적백분비,Nissil염색,광경하관찰해마CA1구병이학결과,진행존활신경원계수,병측정조망지수(AI).결과 여S조비교,기여4조NDS평분、뇌경사체적백분비、AI증가,존활신경원계수강저(P＜0.05);여I/R조비교,RLIP조、EAP조화RLIP+EAP조NDS평분、뇌경사체적백분비화AI강저,존활신경원계수승고(P＜0.05);여RLIP조화EAP조비교,RLIP+EAP조NDS평분、뇌경사체적백분비화AI강저,존활신경원계수승고(P＜0.05).결론 지체원격결혈후처리연합전침후처리감경대서국조성뇌결혈재관주손상적효과우우단독일충방법.
Objective To evaluate the effect of remote limb ischemic postconditioning (RLIP) combined with electro-acupuncture postconditioning (EAP) on focal cerebral ischemia-reperfusion (I/R) injury in rats.Methods Seventy male Sprague-lDawley rats,weighing 220-250 g,were randomly assigned into 5 groups (n =14 each) using a random number table:sham operation group (group S),group I/R,RLIP group,EAP group and RLIP combined with EAP group (group RLIP +EAP).Focal cerebral I/R was induced by middle cerebral artery occlusion (MCAO).MCAO was maintained for 120 min.The animals were subjected to 3 cycles of 15 min ischemia of bilateral hind limbs followed by 30 s reperfusion starting from onset of reperfusion.Dazhui and Baihui acupoints were stimulated with electric stimulator (frequency 15 Hz/2 Hz,intensity 1 mA) for 30 min starting from onset of reperfusion.Neurological deficit scores (NDSs) were evaluated at 24 h of reperfusion.The animals were then sacrificed and brains were removed to measure the infarct size and for microscopic examination of pathological changes in hippocampal CA1 region.The number of survival neurons was counted.The apoptosis in neurons was determined by TUNEL.Apoptosis index was calculated.Results Compared with group S,the NDSs,percentage of cerebral infarct size and AI were significantly increased,and the number of survival neurons was decreased in the other four groups.Compared with group I/R,the NDSs,percentage of cerebral infarct size and AI were significantly decreased,and the number of survival neurons was increased in EAP,RLIP and RLIP+EAP groups.Compared with RLIP and EAP groups,the NDSs,percentage of cerebral infarct size and AI were significantly decreased,and the number of survival neurons was increased in group RLIP+ EAP.Conclusion The combination of RLIP and EAP provides better efficacy than either alone in attenuating focal cerebral I/R injury in rats.
