CAJ | 학술논문

目的评价右美托咪定联合浅低温对新生大鼠全脑缺血再灌注损伤的影响.方法新生SD大鼠96只,日龄6～7d,体重18～22 g.采用随机数字表法,将其分为4组(n=24):缺血再灌注组(I/R组)、浅低温组(H组)、右美托咪定组(D组)、右美托咪定联合浅低温组(DH组).采用夹闭双侧颈总动脉缺血15 min后恢复灌注加低血压制备全脑缺血再灌注损伤模型.D组和DH组于缺血前30 min腹腔注射右美托咪定75μg/kg,I/R组和H组腹腔注射等容量生理盐水.I/R组和D组维持颞肌温度36.7～37.2℃,H组和DH组自缺血开始,维持颞肌温度34.8～35.3 ℃ 2 h.于再灌注12、24和72 h(T1-3)时每组随机取8只大鼠行神经功能缺陷评分(NDS评分),然后处死大鼠取脑组织,采用分光光度法确定脑组织髓过氧化物酶(MPO)活性,采用ELISA法测定脑组织TNF-α、IL-6含量.结果与I/R组比较,其余3组NDS评分、MPO活性、IL-6和TNF-α含量降低(P＜0.05);与H组及D组比较,DH组NDS评分、MPO活性、IL-6和TNF-α含量降低(P＜0.05).结论右美托咪定通过抑制脑组织炎性反应,可优化浅低温对脑缺血再灌注新生大鼠的脑保护作用.
목적 평개우미탁미정연합천저온대신생대서전뇌결혈재관주손상적영향.방법 신생SD대서96지,일령6～7d,체중18～22 g.채용수궤수자표법,장기분위4조(n=24):결혈재관주조(I/R조)、천저온조(H조)、우미탁미정조(D조)、우미탁미정연합천저온조(DH조).채용협폐쌍측경총동맥결혈15 min후회복관주가저혈압제비전뇌결혈재관주손상모형.D조화DH조우결혈전30 min복강주사우미탁미정75μg/kg,I/R조화H조복강주사등용량생리염수.I/R조화D조유지섭기온도36.7～37.2℃,H조화DH조자결혈개시,유지섭기온도34.8～35.3 ℃ 2 h.우재관주12、24화72 h(T1-3)시매조수궤취8지대서행신경공능결함평분(NDS평분),연후처사대서취뇌조직,채용분광광도법학정뇌조직수과양화물매(MPO)활성,채용ELISA법측정뇌조직TNF-α、IL-6함량.결과 여I/R조비교,기여3조NDS평분、MPO활성、IL-6화TNF-α함량강저(P＜0.05);여H조급D조비교,DH조NDS평분、MPO활성、IL-6화TNF-α함량강저(P＜0.05).결론 우미탁미정통과억제뇌조직염성반응,가우화천저온대뇌결혈재관주신생대서적뇌보호작용.
Objective To evaluate the effects of combination of dexmedetomidine and mild hypothermia on global cerebral ischemia-reperfusion (I/R) injury in neonatal rats.Methods Ninety-six neonatal Sprague-Dawley rats,aged 6-7 days,weighing 18-22 g,were randomly divided into 4 groups (n=24 each) using a random number table:I/R group,mild hypothermia group (group H),dexmedetomidine group (group D) and combination of dexmedetomidine and mild hypothermia group (group DH).Global cerebral ischemia was induced in rats anaesthetized with chloral hydrate by bilateral common carotid artery clamping (for 15 min) combined with hypotension followed by reperfusion.Dexmedetomidine 75 pg/kg was given intraperitoneally at 30 min before ischemia in D and DH groups,while the equal volume of normal saline was given in I/R and H groups.The temperature in the temporal muscle was maintained at 36.7-37.2℃ in I/R and D groups,and at 34.8-35.3℃ in H and DH groups.At 12,24 and 72 h of reperfusion,8 rats were randomly chosen in each group,and neurological deficit score (NDS) was determined.The animals were then sacrificed,and their brains were removed for determination of myeloperoxidase (MPO) activity (by spectrophotometry) and contents of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in brain tissues (using ELISA).Results Compared with I/R group,the NDS,MPO activity and contents of TNF-α and IL-6 were significantly decreased in the other three groups.The NDS,MPO activity and contents of TNF-α and IL-6 were significantly lower in DH group than in H or D group.Conclusion Dexmedetomidine can optimize cerebral protection providedby mild hypothermia against global cerebral I/R injury through inhibiting inflammatory responses in brain tissues of neonatal rats.