CAJ | 학술논문

目的：??利用差异蛋白质组学技术检测十肽对变异链球菌蛋白的作用反应，初步鉴定并验证变异链球菌致龋过程中的部分重要蛋白质。方法??人工合成十肽（氨基酸序列：KKVVFKVKFK-NH2），利用双向电泳技术分离十肽作用前后变异链球菌的总蛋白，采用质谱分析联合生物信息学技术鉴定十肽作用前后发生差异性改变的变异链球菌蛋白质，并在蛋白质水平验证关键差异蛋白质烯醇酶的表达情况。结果??十肽作用前后变异链球菌蛋白质发生明显变化，其中发生差异性改变的蛋白质功能主要包括降解碳水化合物（经糖酵解途径），蛋白质折叠、结合、运输及蛋白质翻译等。Western?blot验证结果显示，十肽处理后烯醇酶的表达量明显降低。结论??人工合成抗菌肽十肽处理前后变异链球菌的蛋白质表达发生明显变化，推测其可能通过改变烯醇酶等标志性蛋白的表达而达到防龋作用。
목적：??이용차이단백질조학기술검측십태대변이련구균단백적작용반응，초보감정병험증변이련구균치우과정중적부분중요단백질。방법??인공합성십태（안기산서렬：KKVVFKVKFK-NH2），이용쌍향전영기술분리십태작용전후변이련구균적총단백，채용질보분석연합생물신식학기술감정십태작용전후발생차이성개변적변이련구균단백질，병재단백질수평험증관건차이단백질희순매적표체정황。결과??십태작용전후변이련구균단백질발생명현변화，기중발생차이성개변적단백질공능주요포괄강해탄수화합물（경당효해도경），단백질절첩、결합、운수급단백질번역등。Western?blot험증결과현시，십태처리후희순매적표체량명현강저。결론??인공합성항균태십태처리전후변이련구균적단백질표체발생명현변화，추측기가능통과개변희순매등표지성단백적표체이체도방우작용。
Objective??To?compare?the?protein?profiles?between?decapeptide-treated?and?untreated?planktonic?cells?of?Streptococcus mutans?(S.mutans)?by?differential?proteomic?analysis?to?determine?and?identify?the?key?proteins.Methods??In?our?previous?study,?we?investigated?decapeptide?(KKVVFKVKFK–NH2),?which?was?a?novel?adenosine?monophosphate.?Com-pared?with?other?oral?pathogens?tested,?decapeptide?had?a?preferential?antibacterial?activity?against?S. mutans.?It?also?inhibited?S. mutans?biofilm?formation?and?reduced?the?one-day?developed?biofilm.?In?the?present?study,?we?first?synthesized?decapep-tide,?and?then?compared?the?protein?profiles?between?decapeptide-treated?and?untreated?planktonic?cells?of?S. mutans?by?two-dimensional?gel?electrophoresis?and?matrix-assisted?laser?desorption?ionization?time-of-flight?mass?spectrometry.?We?also?verified?different?expressions?of?key?protein?enolase?in?the?protein?level.?Results??The?results?showed?that?decapeptide?altered?the?protein?expression?of?planktonic?S. mutans.?These?proteins?were?functionally?involved?in?carbohydrate?degradation?by?gly-colysis,?protein?folding,?conjunction,?transport,?translation,?adenosine?triphosphate?binding,?protein?binding,?sequence-specific?DNA?binding,?transcription?factor?activity,?and?two-component?response?regulator?activity.?Western?blot?results?showed?that?enolase?protein?expression?decreased?obviously?in?decapeptide-treated?cells?of?S. mutans.?Conclusion??The?protein?expression?of?S. mutans?significantly?changed?after?synthetic?antimicrobial?decapeptide?treatment,?suggesting?that?decapeptide?may?pre-sent?a?preferential?effect?on?oral?caries?by?changing?the?expression?of?certain?key?proteins,?such?as?enolase?protein.