CAJ | 학술논문

以长期驯化选育得到的耐抑制物氧化葡萄糖酸杆菌Glucobacter oxydans NL71为催化菌株,对全细胞直接催化木质纤维(麦秆)稀酸预处理水解液产木糖酸的工艺进行研究。结果表明：未脱毒水解液对催化反应生产木糖酸具有抑制作用,且随浓度提高,抑制作用增强。提高细胞浓度和提高供氧能力可以有效抵抗麦杆稀水解液的抑制效应。在摇瓶体系中以2 g/L细胞接种量直接催化未经脱毒的麦秆稀酸水解浓缩液,当初始木糖质量浓度超过100 g/L时,细胞的催化性能受到严重抑制,木糖酸得率均低于50%；当细胞接种量提升至8 g/L时,木糖酸得率可以达到85.7%；在机械搅拌式通氧加压反应体系中,采用密封加氧技术对100 g/L木糖浓度的麦秸稀酸水解浓缩液全细胞催化24 h的细胞接种量8 g/L,木糖酸终质量浓度达到103.1 g/L,木糖酸得率可达到93.1%,产生速率为摇瓶体系的1.6倍。
이장기순화선육득도적내억제물양화포도당산간균Glucobacter oxydans NL71위최화균주,대전세포직접최화목질섬유(맥간)희산예처리수해액산목당산적공예진행연구。결과표명：미탈독수해액대최화반응생산목당산구유억제작용,차수농도제고,억제작용증강。제고세포농도화제고공양능력가이유효저항맥간희수해액적억제효응。재요병체계중이2 g/L세포접충량직접최화미경탈독적맥간희산수해농축액,당초시목당질량농도초과100 g/L시,세포적최화성능수도엄중억제,목당산득솔균저우50%；당세포접충량제승지8 g/L시,목당산득솔가이체도85.7%；재궤계교반식통양가압반응체계중,채용밀봉가양기술대100 g/L목당농도적맥갈희산수해농축액전세포최화24 h적세포접충량8 g/L,목당산종질량농도체도103.1 g/L,목당산득솔가체도93.1%,산생속솔위요병체계적1.6배。
The production of xylonic acid from hydrolysates of straw by whole-cell catalysis was investigated with long-term domesticated Gluconobacter oxydans as catalytic strain. The results showed that the catalytic reaction could be inhibited by hydrolysates without detoxication,and the inhibitory effect was more obvious with the increase of concentration. However,it could be counteracted by increasing inoculation quantity and dissolved oxygen. In the shake flask system,with the inoculation amount of 2 g/L and the xylose concentration of hydrolysates more than 100 g/L,the performance of cell catalysis was inhibited seriously, and the yield of xylonic acid and utilization rate of xylose were all lower than 50%. When the inoculation amount increased to 8 g/L,the yield of xylonic acid reached 85. 7%. The method of compressed oxygen supply in the sealed aerated stirred tank reactor was investigated,and in 24 h the concentration and yield of xylonic acid reached 103. 1 g/L and 93. 1%,respectively. And the production rate increased 1. 6 folds compared with that of the shaker system when xylose concentration of hydrolysates was 100 g/L and inoculation amount of G. oxydans was 8 g/L.