CAJ | 학술논문

采用RT‐PCR和RACE技术从芙蓉李分离得到1条长度为1309 bp的 PsACO1基因cDNA全长序列。该基因包含长度为957 bp的开放阅读框，编码319个氨基酸。PsACO1蛋白的分子量为36.24 kD ，等电点为5.28，含有9个ACO特有的保守结构域。系统进化分析结果表明PsACO1与碧桃、梅、枇杷、苹果和欧洲李的ACO蛋白的亲缘关系较近。
채용RT‐PCR화RACE기술종부용리분리득도1조장도위1309 bp적 PsACO1기인cDNA전장서렬。해기인포함장도위957 bp적개방열독광，편마319개안기산。PsACO1단백적분자량위36.24 kD ，등전점위5.28，함유9개ACO특유적보수결구역。계통진화분석결과표명PsACO1여벽도、매、비파、평과화구주리적ACO단백적친연관계교근。
A 1309 bp full length cDNA sequence of PsACO1 gene were isolated from Furong plum (Prunus salicina Lindl.) through RT‐PCR and RACE technology. The isolated gene contains a 957 bp open reading frame and encodes a protein of 319 amino acids with isoelectric point of 5.28 and molecular weight of 36.24 kD. PsACO1 protein contains characteristic domains of ACO proteins. The phylogenetic analysis results showed that PsACO 1 has close corelation with ACO protein from Prunus persica ,Prunus mume ,Eriobotrya japonica ,Malus domestica and Prunus domestica .