中华耳科学杂志
中華耳科學雜誌
중화이과학잡지
CHINESE JOURNAL OF OTOLOGY
2015年
1期
171-175
,共5页
CRISPR/Cas系统%耳聋%动物模型
CRISPR/Cas繫統%耳聾%動物模型
CRISPR/Cas계통%이롱%동물모형
CRISPR/Cas system%Deafness%Animal model
CRISPR( Clustered regularly interspaced short palindromicrepeats)/Cas( CRISPR-associated)系统是新一代的靶向基因组编辑技术系统,其突出特征是Cas蛋白,其通过gRNA( guide RNA)的引导下对外源性的DNA进行靶向切割,从而为细菌入侵核酸提供了免疫力。由CRISPR/Cas系统介导的基因靶向修饰技术成功地应用于多种细菌及多种生物的基因组靶向修饰,对人类基因组DNA的靶向修饰也已经得到了证实,并且CRISPR/Cas系统与以往技术相比在很多方面显示出了的优点。CRISPR/Cas系统在遗传缺陷疾病动物模型的建立技术中已经占有重要地位,而各种类型基因修饰动物模型是目前内耳基因功能、聋病分子机制研究的最理想方法和必备条件。随着耳聋的遗传学研究不断深入,诊断水平的不断提高,对遗传性耳聋动物模型的需求也不断增多,CRISPR/Cas系统介导的基因组靶向修饰技术也会不断完善,该技术将在耳聋的遗传学研究和诊断治疗等多领域发挥更大作用。
CRISPR( Clustered regularly interspaced short palindromicrepeats)/Cas( CRISPR-associated)繫統是新一代的靶嚮基因組編輯技術繫統,其突齣特徵是Cas蛋白,其通過gRNA( guide RNA)的引導下對外源性的DNA進行靶嚮切割,從而為細菌入侵覈痠提供瞭免疫力。由CRISPR/Cas繫統介導的基因靶嚮脩飾技術成功地應用于多種細菌及多種生物的基因組靶嚮脩飾,對人類基因組DNA的靶嚮脩飾也已經得到瞭證實,併且CRISPR/Cas繫統與以往技術相比在很多方麵顯示齣瞭的優點。CRISPR/Cas繫統在遺傳缺陷疾病動物模型的建立技術中已經佔有重要地位,而各種類型基因脩飾動物模型是目前內耳基因功能、聾病分子機製研究的最理想方法和必備條件。隨著耳聾的遺傳學研究不斷深入,診斷水平的不斷提高,對遺傳性耳聾動物模型的需求也不斷增多,CRISPR/Cas繫統介導的基因組靶嚮脩飾技術也會不斷完善,該技術將在耳聾的遺傳學研究和診斷治療等多領域髮揮更大作用。
CRISPR( Clustered regularly interspaced short palindromicrepeats)/Cas( CRISPR-associated)계통시신일대적파향기인조편집기술계통,기돌출특정시Cas단백,기통과gRNA( guide RNA)적인도하대외원성적DNA진행파향절할,종이위세균입침핵산제공료면역력。유CRISPR/Cas계통개도적기인파향수식기술성공지응용우다충세균급다충생물적기인조파향수식,대인류기인조DNA적파향수식야이경득도료증실,병차CRISPR/Cas계통여이왕기술상비재흔다방면현시출료적우점。CRISPR/Cas계통재유전결함질병동물모형적건립기술중이경점유중요지위,이각충류형기인수식동물모형시목전내이기인공능、롱병분자궤제연구적최이상방법화필비조건。수착이롱적유전학연구불단심입,진단수평적불단제고,대유전성이롱동물모형적수구야불단증다,CRISPR/Cas계통개도적기인조파향수식기술야회불단완선,해기술장재이롱적유전학연구화진단치료등다영역발휘경대작용。
The CRISPR (Clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system is a new generation of targeted genome editing technique system. Its prominent feature is the Cas protein, which is capable of targeted cutting of exogenous DNA under the guidance of gRNA (guide RNA) and provides immunity to bacteria invasion of nu?cleic acids. CRISPR/Cas system,mediated targeted gene modification technology has been successfully has been applied to a variety of bacteria and other biological genomes, as well as to human genomic DNA. Compared previous technology, the CRIS?PR/Cas system has shown a number of advantages on numerous aspects. The CRISPR/Cas system is now an important techni?cal means in establishing animal model of genetic defect diseases. Various types of genetically modified animal models are a preferred approach and essential in studying inner ear gene functions and molecular mechanisms of deafness. Deafness gene studies advance and diagnosis improves, there will be increasing needs for animal models of hereditary deafness. CRISPR/Cas system mediated targeted the genome modification technology will continue to improve and will play an increasingly important role in many fields of genetic research and diagnosis and treatment of deafness.