中华耳科学杂志
中華耳科學雜誌
중화이과학잡지
CHINESE JOURNAL OF OTOLOGY
2015年
1期
156-160
,共5页
HO-1%阿魏酸%豚鼠%螺旋神经元
HO-1%阿魏痠%豚鼠%螺鏇神經元
HO-1%아위산%돈서%라선신경원
HO-1%Ferulic acid%Guinea pig%Spiral neurons
目的:检测血红素加氧酶-1在豚鼠耳蜗螺旋神经节顺铂损伤后的表达变化。方法通过免疫组织化学及Western Blot技术,观察耳蜗螺旋神经元损伤后HO-1在不同组间的表达变化。将32只健康豚鼠随机分为4组,每组8只。A组对照组:按体重以0.9%生理盐水12ml/kg腹腔注射,早晚各一次,持续3天。B组顺铂组:按体重12mg/kg顺铂腹腔注射(IP),早晚各一次,持续3天。C组阿魏酸(FA)+顺铂组:先150mg/kgFA腹腔注射预处理1h,再以12mg/kg顺铂IP,早晚各一次,持续三天。D组FA+锌原卟啉Ⅸ(ZnppⅨ)+顺铂组:同时给予150mg/kgFA+ZnppⅨ15umol/kg IP预处理1h,再以12mg/kg顺铂IP,早晚各一次,持续三天。采用免疫组织化学及Wstern Blot技术检测不同组间螺旋神经元HO-1蛋白表达变化。结果在不同的分组中,HO-1在耳蜗螺旋神经节中表达量不同。空白组中,螺旋神经节组织存在微弱的HO-1表达,其蛋白表达量为0.48±0.07;A组蛋白表达量为0.81±0.07,B组蛋白表达量为0.9±0.05,C组蛋白表达量为0.61±0.07。4个组两两间差异均有统计学意义(p<0.0001)。结论 HO-1在C组中表达量最多,B组次之,D组最少,表明HO-1可能参与顺铂损伤螺旋神经元后的修复过程。
目的:檢測血紅素加氧酶-1在豚鼠耳蝸螺鏇神經節順鉑損傷後的錶達變化。方法通過免疫組織化學及Western Blot技術,觀察耳蝸螺鏇神經元損傷後HO-1在不同組間的錶達變化。將32隻健康豚鼠隨機分為4組,每組8隻。A組對照組:按體重以0.9%生理鹽水12ml/kg腹腔註射,早晚各一次,持續3天。B組順鉑組:按體重12mg/kg順鉑腹腔註射(IP),早晚各一次,持續3天。C組阿魏痠(FA)+順鉑組:先150mg/kgFA腹腔註射預處理1h,再以12mg/kg順鉑IP,早晚各一次,持續三天。D組FA+鋅原卟啉Ⅸ(ZnppⅨ)+順鉑組:同時給予150mg/kgFA+ZnppⅨ15umol/kg IP預處理1h,再以12mg/kg順鉑IP,早晚各一次,持續三天。採用免疫組織化學及Wstern Blot技術檢測不同組間螺鏇神經元HO-1蛋白錶達變化。結果在不同的分組中,HO-1在耳蝸螺鏇神經節中錶達量不同。空白組中,螺鏇神經節組織存在微弱的HO-1錶達,其蛋白錶達量為0.48±0.07;A組蛋白錶達量為0.81±0.07,B組蛋白錶達量為0.9±0.05,C組蛋白錶達量為0.61±0.07。4箇組兩兩間差異均有統計學意義(p<0.0001)。結論 HO-1在C組中錶達量最多,B組次之,D組最少,錶明HO-1可能參與順鉑損傷螺鏇神經元後的脩複過程。
목적:검측혈홍소가양매-1재돈서이와라선신경절순박손상후적표체변화。방법통과면역조직화학급Western Blot기술,관찰이와라선신경원손상후HO-1재불동조간적표체변화。장32지건강돈서수궤분위4조,매조8지。A조대조조:안체중이0.9%생리염수12ml/kg복강주사,조만각일차,지속3천。B조순박조:안체중12mg/kg순박복강주사(IP),조만각일차,지속3천。C조아위산(FA)+순박조:선150mg/kgFA복강주사예처리1h,재이12mg/kg순박IP,조만각일차,지속삼천。D조FA+자원계람Ⅸ(ZnppⅨ)+순박조:동시급여150mg/kgFA+ZnppⅨ15umol/kg IP예처리1h,재이12mg/kg순박IP,조만각일차,지속삼천。채용면역조직화학급Wstern Blot기술검측불동조간라선신경원HO-1단백표체변화。결과재불동적분조중,HO-1재이와라선신경절중표체량불동。공백조중,라선신경절조직존재미약적HO-1표체,기단백표체량위0.48±0.07;A조단백표체량위0.81±0.07,B조단백표체량위0.9±0.05,C조단백표체량위0.61±0.07。4개조량량간차이균유통계학의의(p<0.0001)。결론 HO-1재C조중표체량최다,B조차지,D조최소,표명HO-1가능삼여순박손상라선신경원후적수복과정。
Objective To investigate effects of heme oxygenase-1(HO-1) on spiral ganglion cells neurons (SGNs) in cis?platin-induced injury. Methods Forty adult guinea pigs were divided randomly into 4 groups (n=8 in each group) to receive intraperitoneal injection of 0.9%saline (Group A), or cisplatin (Group B), or ferulic acid (150 mg/kg, bid) and cisplatin (Group C), or ferulic acid (150 mg/kg, bid), zinc-protoporphyrin-Ⅸ(15 umol/kg, bid) and cisplatin (Group D), all at 12 ml/kg, bid for 3 days. HO-1 expressing pattern in damaged cochlea was studied by immunochemistry and Western Blot techniques. Results in Group A, faint HO-1 expression was seen in the spiral ganglion tissue with its protein expression only at 0.48 ± 0.07. HO-1 protein expression was 0.81 ± 0.07 in Group B, 0.9 ± 0.05 in Group C and 0.61 ± 0.07 in Group D. The differences in HO-1 pro?tein expression between the four groups were statistically significant (p<0.0001). Conclusion Among animals exposed to cispl?atin, expression of HO-1 was the highest in Group C and the lowest in Group D, indicating possible HO-1 involvement in the re?pair process in spiral neurons following damage by cisplatin.