CAJ | 학술논문

目的：检测血红素加氧酶-1在豚鼠耳蜗螺旋神经节顺铂损伤后的表达变化。方法通过免疫组织化学及Western Blot技术，观察耳蜗螺旋神经元损伤后HO-1在不同组间的表达变化。将32只健康豚鼠随机分为4组，每组8只。A组对照组：按体重以0.9%生理盐水12ml/kg腹腔注射，早晚各一次，持续3天。B组顺铂组：按体重12mg/kg顺铂腹腔注射（IP），早晚各一次，持续3天。C组阿魏酸（FA）+顺铂组：先150mg/kgFA腹腔注射预处理1h，再以12mg/kg顺铂IP，早晚各一次，持续三天。D组FA+锌原卟啉Ⅸ（ZnppⅨ）+顺铂组：同时给予150mg/kgFA+ZnppⅨ15umol/kg IP预处理1h，再以12mg/kg顺铂IP，早晚各一次，持续三天。采用免疫组织化学及Wstern Blot技术检测不同组间螺旋神经元HO-1蛋白表达变化。结果在不同的分组中，HO-1在耳蜗螺旋神经节中表达量不同。空白组中，螺旋神经节组织存在微弱的HO-1表达，其蛋白表达量为0.48±0.07；A组蛋白表达量为0.81±0.07，B组蛋白表达量为0.9±0.05,C组蛋白表达量为0.61±0.07。4个组两两间差异均有统计学意义（p<0.0001）。结论 HO-1在C组中表达量最多，B组次之，D组最少，表明HO-1可能参与顺铂损伤螺旋神经元后的修复过程。
목적：검측혈홍소가양매-1재돈서이와라선신경절순박손상후적표체변화。방법통과면역조직화학급Western Blot기술，관찰이와라선신경원손상후HO-1재불동조간적표체변화。장32지건강돈서수궤분위4조，매조8지。A조대조조：안체중이0.9%생리염수12ml/kg복강주사，조만각일차，지속3천。B조순박조：안체중12mg/kg순박복강주사（IP），조만각일차，지속3천。C조아위산（FA）+순박조：선150mg/kgFA복강주사예처리1h，재이12mg/kg순박IP，조만각일차，지속삼천。D조FA+자원계람Ⅸ（ZnppⅨ）+순박조：동시급여150mg/kgFA+ZnppⅨ15umol/kg IP예처리1h，재이12mg/kg순박IP，조만각일차，지속삼천。채용면역조직화학급Wstern Blot기술검측불동조간라선신경원HO-1단백표체변화。결과재불동적분조중，HO-1재이와라선신경절중표체량불동。공백조중，라선신경절조직존재미약적HO-1표체，기단백표체량위0.48±0.07；A조단백표체량위0.81±0.07，B조단백표체량위0.9±0.05,C조단백표체량위0.61±0.07。4개조량량간차이균유통계학의의（p<0.0001）。결론 HO-1재C조중표체량최다，B조차지，D조최소，표명HO-1가능삼여순박손상라선신경원후적수복과정。
Objective To investigate effects of heme oxygenase-1(HO-1) on spiral ganglion cells neurons (SGNs) in cis?platin-induced injury. Methods Forty adult guinea pigs were divided randomly into 4 groups (n=8 in each group) to receive intraperitoneal injection of 0.9%saline (Group A), or cisplatin (Group B), or ferulic acid (150 mg/kg, bid) and cisplatin (Group C), or ferulic acid (150 mg/kg, bid), zinc-protoporphyrin-Ⅸ(15 umol/kg, bid) and cisplatin (Group D), all at 12 ml/kg, bid for 3 days. HO-1 expressing pattern in damaged cochlea was studied by immunochemistry and Western Blot techniques. Results in Group A, faint HO-1 expression was seen in the spiral ganglion tissue with its protein expression only at 0.48 ± 0.07. HO-1 protein expression was 0.81 ± 0.07 in Group B, 0.9 ± 0.05 in Group C and 0.61 ± 0.07 in Group D. The differences in HO-1 pro?tein expression between the four groups were statistically significant (p<0.0001). Conclusion Among animals exposed to cispl?atin, expression of HO-1 was the highest in Group C and the lowest in Group D, indicating possible HO-1 involvement in the re?pair process in spiral neurons following damage by cisplatin.