实用中医药杂志
實用中醫藥雜誌
실용중의약잡지
JOURNAL OF PRACTICAL TRADITIONAL CHINESE MEDICINE
2015年
4期
268-269,270
,共3页
唐思诗%唐念珍%唐成林%杨辉%张毅%田源
唐思詩%唐唸珍%唐成林%楊輝%張毅%田源
당사시%당념진%당성림%양휘%장의%전원
胰岛素抵抗%大鼠模型%电针%ERK%JNK
胰島素牴抗%大鼠模型%電針%ERK%JNK
이도소저항%대서모형%전침%ERK%JNK
Insulin resistant%Rat model%electroacupuncture%ERK%JNK
目的：探讨电针对胰岛素抵抗大鼠骨骼肌ERK、JNK蛋白表达的影响。方法：将SD雄性大鼠随机分为空白组、模型1组、模型2组、电针1组、电针2组，用高脂高糖饮食喂养法建立胰岛素抵抗大鼠模型，用葡萄糖氧化酶法检测FBG、放射免疫分析法检测FINS，并计算Homa-IR。确定造模成功后，电针组大鼠取足三里、三阴交、胃脘下俞行电针治疗，用Western blot技术检测大鼠骨骼肌中ERK、JNK蛋白的表达。结果：模型组大鼠FBG、FINS、Homa-IR较空白组显著升高（P＜0.05），电针组大鼠FBG、FINS、Homa-IR较模型组显著下降（P＜0.05），模型组大鼠ERK、JNK蛋白表达较空白组显著上升（P＜0.05），电针组大鼠ERK、JNK蛋白表达较模型组显著下降（P＜0.05）。结论：电针可降低ERK、JNK蛋白表达水平，从而改善胰岛素抵抗状态。
目的：探討電針對胰島素牴抗大鼠骨骼肌ERK、JNK蛋白錶達的影響。方法：將SD雄性大鼠隨機分為空白組、模型1組、模型2組、電針1組、電針2組，用高脂高糖飲食餵養法建立胰島素牴抗大鼠模型，用葡萄糖氧化酶法檢測FBG、放射免疫分析法檢測FINS，併計算Homa-IR。確定造模成功後，電針組大鼠取足三裏、三陰交、胃脘下俞行電針治療，用Western blot技術檢測大鼠骨骼肌中ERK、JNK蛋白的錶達。結果：模型組大鼠FBG、FINS、Homa-IR較空白組顯著升高（P＜0.05），電針組大鼠FBG、FINS、Homa-IR較模型組顯著下降（P＜0.05），模型組大鼠ERK、JNK蛋白錶達較空白組顯著上升（P＜0.05），電針組大鼠ERK、JNK蛋白錶達較模型組顯著下降（P＜0.05）。結論：電針可降低ERK、JNK蛋白錶達水平，從而改善胰島素牴抗狀態。
목적：탐토전침대이도소저항대서골격기ERK、JNK단백표체적영향。방법：장SD웅성대서수궤분위공백조、모형1조、모형2조、전침1조、전침2조，용고지고당음식위양법건립이도소저항대서모형，용포도당양화매법검측FBG、방사면역분석법검측FINS，병계산Homa-IR。학정조모성공후，전침조대서취족삼리、삼음교、위완하유행전침치료，용Western blot기술검측대서골격기중ERK、JNK단백적표체。결과：모형조대서FBG、FINS、Homa-IR교공백조현저승고（P＜0.05），전침조대서FBG、FINS、Homa-IR교모형조현저하강（P＜0.05），모형조대서ERK、JNK단백표체교공백조현저상승（P＜0.05），전침조대서ERK、JNK단백표체교모형조현저하강（P＜0.05）。결론：전침가강저ERK、JNK단백표체수평，종이개선이도소저항상태。
Objective:To investigate the influence of electroacupuncture on expression of ERK and JNK protein in the skeletal muscle of insulin resistant rat. Method:The male SD rats were randomly divided into five groups, blank group,model 1 group,model 2 group,EA 1 group and EA 2 group. To establish the insulin resistant rats model by feeding with high fat and high sugar diet. We detect FBG with the glucose oxidase method and FINS with the radioimmunoassay,and then we calculated the Homa-IR. After the successful modeling determined,the EA group were treated with electroacupuncture at Zusanli,Sanyinjiao and Weiwanxiashu. The expression of ERK and JNK protein in the skeletal muscle of rats was detected with the Western blot. Result:After electroacupuncture,the FBG, FINS and Homa-IR of each model group significantly increased more than that of blank l group(P<0.05). The expression of ERK and JNK protein of each model group significantly increased more than that of blank l group(P<0.05).The FBG, FINS, Homa-IR of each EA group significantly decreased more than that of each model group (P<0.05). The expression of ERK and JNK protein of each EA group significantly decreased more than that of each model group(P<0.05).Conclusion:The electroacupuncture could improve insulin resistant by regulating the expression level of ERK and JNK protein in the insulin resistant rat.
