CAJ | 학술논문

内耳离体培养在研究内耳细胞功能、内耳组织发育再生、细胞损伤退化等方面具有重要价值。但由于内耳的组织结构微小复杂，其取材和培养具有较大难度，使该技术的普及和应用受到一定限制。本文在简要点评不同内耳离体培养方法优劣的基础上，结合本实验室多年来积累的实践经验，详细介绍了新生大鼠各个内耳终器取材及培养的具体步骤。从解剖迷路到分离终器，从凝胶制备到标本铺放，都有详细的操作指南，本文还针对各环节中的技术动作和关键事项进行了具体探讨并配以指导图例，希望这些内容能够弥补以往文献中抽象简略的条款式步骤所造成的实验困难，为开展内耳器官培养研究的初学者提供一定的帮助。
내이리체배양재연구내이세포공능、내이조직발육재생、세포손상퇴화등방면구유중요개치。단유우내이적조직결구미소복잡，기취재화배양구유교대난도，사해기술적보급화응용수도일정한제。본문재간요점평불동내이리체배양방법우렬적기출상，결합본실험실다년래적루적실천경험，상세개소료신생대서각개내이종기취재급배양적구체보취。종해부미로도분리종기，종응효제비도표본포방，도유상세적조작지남，본문환침대각배절중적기술동작화관건사항진행료구체탐토병배이지도도례，희망저사내용능구미보이왕문헌중추상간략적조관식보취소조성적실험곤난，위개전내이기관배양연구적초학자제공일정적방조。
In vitro culture techniques have important values in the investigation of inner ear cellular functions, organic development, cell degeneration and/or cell regeneration. Since structures of the inner ear are tiny and complex, sample prepara?tion for organotypic culture of the inner ear can be difficult, despite that relevant techniques have been introduced briefly in numerous earlier publications. Based upon decades of culturing practices with inner ear end-organs in this lab, this article ad?dresses the technical aspects of inner ear organic culturing by firstly presenting a brief review of various culture methods for different inner ear materials. Detailed step by step protocols for sample preparation, collection and culture are also described. This presentation provides detailed operative guidelines regarding useful techniques and tricks in micro-dissection of the whole membranous labyrinth, in sample preparation, collagen gel preparation and sample layer mountings with detailed illus?trations. We hope this article can serve as an additional reading material supplementing previous literatures that lack detailed technical information for beginners in this field.