CAJ | 학술논문

根据GenBank中已发表的鸢尾轻花叶病毒（Iris mild mosaic virus ，IMMV）、水仙潜隐病毒（Narcissus latent virus ，NLV）和菜豆黄花叶病毒（Bean yellow mosaic virus ，BYMV）外壳蛋白（CP）基因序列保守区域分别设计特异性引物，通过优化多重PCR反应条件，建立能同时检测球根鸢尾3种病毒的多重PCR检测体系。该体系能够一次扩增出IMMV、NLV和BYMV的特异片段，其大小分别是770 bp、266 bp和186 bp。测序结果表明，3种病毒序列与相应的参考序列相似性均达98％以上。灵敏度测定结果表明，从相当于或大于10－2 m g的感病植物组织中能够检测到这3种病毒。
근거GenBank중이발표적연미경화협병독（Iris mild mosaic virus ，IMMV）、수선잠은병독（Narcissus latent virus ，NLV）화채두황화협병독（Bean yellow mosaic virus ，BYMV）외각단백（CP）기인서렬보수구역분별설계특이성인물，통과우화다중PCR반응조건，건립능동시검측구근연미3충병독적다중PCR검측체계。해체계능구일차확증출IMMV、NLV화BYMV적특이편단，기대소분별시770 bp、266 bp화186 bp。측서결과표명，3충병독서렬여상응적삼고서렬상사성균체98％이상。령민도측정결과표명，종상당우혹대우10－2 m g적감병식물조직중능구검측도저3충병독。
By using specific primers designed on the basis of conserved sequences of coat protein (CP) gene ,a multiplex PCR protocol for the detection of three main viruses (Iris mild mosaic virus ,IMMV ;Narcissus latent virus ,NLV ;Bean yellow mosaic virus ,BYMV) in Bulbous Iris was developed. Three specific fragments were simultaneously amplified in one PCR reaction system. Their molecular weights were 770 bp ,266 bp and186 bp respectively. Sequence analysis indicated that the three virus sequences shared at least 98 percent identities with that of other relative viruses. The result showed that the sensitivity of this protocol equaled to be able to detect the three viruses at the level of 10-2 mg Bulbous Iris tissue.