CAJ | 학술논문

采用急性毒性试验和D‐半乳糖氧化损伤小鼠模型试验研究富硒秀珍菇菌粉的急性毒性和对D‐半乳糖致氧化损伤小鼠的抗氧化作用。急性毒性试验结果表明，富硒秀珍菇菌粉对雌雄小鼠经口最大耐受量（MTD）均大于20.0g · kg －1，初步评价认为，富硒秀珍菇菌粉不存在急性毒性风险。D‐半乳糖氧化损伤小鼠模型试验表明，与模型对照组比，富硒秀珍菇菌粉三组的丙二醛（MDA）和蛋白质羰基含量均明显降低（P＜0.01），低、中、高剂量组的 MDA 分别降低20.08％、22.19％、39.26％，蛋白质羰基分别降低38.31％、44.16％和25.32％；富硒秀珍菇菌粉3个剂量组的谷胱甘肽过氧化物酶（GS H‐Px ）活力与模型对照组比均提高，其中，中剂量组和高剂量组均显著提高18.50％左右（ P＜0.05）。值得特别注意的是，与空白对照比，富硒秀珍菇菌粉3个剂量组的MDA、蛋白质羰基、GSH‐Px没有显著差异；说明富硒秀珍菇菌粉对氧化损伤的修复效果基本达到空白对照小鼠水平。
채용급성독성시험화D‐반유당양화손상소서모형시험연구부서수진고균분적급성독성화대D‐반유당치양화손상소서적항양화작용。급성독성시험결과표명，부서수진고균분대자웅소서경구최대내수량（MTD）균대우20.0g · kg －1，초보평개인위，부서수진고균분불존재급성독성풍험。D‐반유당양화손상소서모형시험표명，여모형대조조비，부서수진고균분삼조적병이철（MDA）화단백질탄기함량균명현강저（P＜0.01），저、중、고제량조적 MDA 분별강저20.08％、22.19％、39.26％，단백질탄기분별강저38.31％、44.16％화25.32％；부서수진고균분3개제량조적곡광감태과양화물매（GS H‐Px ）활력여모형대조조비균제고，기중，중제량조화고제량조균현저제고18.50％좌우（ P＜0.05）。치득특별주의적시，여공백대조비，부서수진고균분3개제량조적MDA、단백질탄기、GSH‐Px몰유현저차이；설명부서수진고균분대양화손상적수복효과기본체도공백대조소서수평。
To investigate acute toxicity and antioxidant capacity ,seleniferous Pleurotus geesteranus mycelia (SP) were given to groups of ICR mice and D‐galactose induced oxidative damaged mice , respectively . The maximal tolerance dose (MTD) in SP for mice was greater than 20.0 g · kg -1 body weight ,which defined SP no risk in acute toxicity .Comparing with model control group (CK‐M ) , the MDA and protein carbonyl in mouse serum were decreased significantly by 20.08% ,22.19% ,39.26% and 38.31% ,44.16% ,25.32% for SP‐L ,SP‐M ,SP‐H groups ,respectively ;while GSH‐Px of mice in three SP groups was increased (P<0.05) ,especially in SP‐M and SP‐H groups for 18.5% .However ,there were no significant differences between three SP groups with CK group in serum MDA ,protein carbonyl and GSH ‐Px contents .The results indicated that the protective effect of SP might enable in restoration for oxidative damaged mice getting back to normal like those in control group .