目的 研究缺氧诱导因子1α(hypoxia inducible factor-1α,HIF-1 α)在慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)中的表达及与黏蛋白MUC5AC、MUC5B的关系.方法 选取23例伴鼻息肉的CRS患者、20例不伴鼻息肉的CRS患者和15例无CRS的正常鼻窦黏膜,分别采用过碘酸-雪夫染色(periodic acid-schiff stain,PAS)检测各组中总黏蛋白的表达,免疫组织化学法(immunohistochemistry,IHC)、半定量反转录聚合酶链反应法(RT-PCR)分别检测HIF-1 α及黏蛋白MUC5AC、MUC5B的表达,免疫印记法(Western Blot法)检测各组中HIF-1α表达情况.以SPSS 16.0软件统计分析HIF-1 α与各黏蛋白mRNA表达的相关性.结果 HIF-1α、MUC5AC、MUC5B、总黏蛋白主要表达于伴或不伴鼻息肉CRS患者的黏膜上皮及腺体内,且较健康对照组明显增多,差异有统计学意义(上皮:t值分别为8.650、9.305,9.382、8.524,7.533、5.417,5.507、5.556,P值均＜0.05;腺体:t值分别为8.285、7.098,6.798、7.308,8.574、7.933,6.798、7.308,P值均＜0.05);伴鼻息肉CRS与不伴鼻息肉CRS组间差异无统计学意义(上皮:t值分别为0.734、0.415、0.572、0.248,P值均＞0.05;腺体:t值分别为0.331、0.662、0.249、0.644,P值均＞0.05).HIF-1α蛋白在伴或不伴鼻息肉CRS组织中较正常鼻窦黏膜显著增高(t值分别为3.522、3.314,P值均＜0.05).HIF-1α、MUC5AC、MUC5B mRNA在伴或不伴鼻息肉CRS患者中相对表达量分别为1.35±0.84、1.36±0.94,0.81±0.54、0.78±0.46,1.13±1.00、1.22±1.02,在健康对照组的相对表达量为0.43±0.34、0.42 ±0.36、0.48±0.42,二者相比差异有统计学意义(t值分别为4.087、4.089,2.519、2.550,2.738、2.955,P值均＜0.05).伴鼻息肉CRS患者HIF-1α与MUC5AC、MUC5B mRNA呈正相关(r值分别为0.474、0.464,P值均＜0.05);不伴鼻息肉的CRS患者HIF-1α与MUC5AC、MUC5B mRNA呈正相关(r值分别为0.477、0.514,P值均＜0.05).结论 HIF-1α、MUC5AC和MUC5B在伴或不伴鼻息肉的CRS患者中表达升高,提示CRS是高黏液性疾病,且HIF-1 0与其中高分泌性的黏蛋白MUC5AC、MUC5B存在密切的关系.
目的 研究缺氧誘導因子1α(hypoxia inducible factor-1α,HIF-1 α)在慢性鼻-鼻竇炎(chronic rhinosinusitis,CRS)中的錶達及與黏蛋白MUC5AC、MUC5B的關繫.方法 選取23例伴鼻息肉的CRS患者、20例不伴鼻息肉的CRS患者和15例無CRS的正常鼻竇黏膜,分彆採用過碘痠-雪伕染色(periodic acid-schiff stain,PAS)檢測各組中總黏蛋白的錶達,免疫組織化學法(immunohistochemistry,IHC)、半定量反轉錄聚閤酶鏈反應法(RT-PCR)分彆檢測HIF-1 α及黏蛋白MUC5AC、MUC5B的錶達,免疫印記法(Western Blot法)檢測各組中HIF-1α錶達情況.以SPSS 16.0軟件統計分析HIF-1 α與各黏蛋白mRNA錶達的相關性.結果 HIF-1α、MUC5AC、MUC5B、總黏蛋白主要錶達于伴或不伴鼻息肉CRS患者的黏膜上皮及腺體內,且較健康對照組明顯增多,差異有統計學意義(上皮:t值分彆為8.650、9.305,9.382、8.524,7.533、5.417,5.507、5.556,P值均＜0.05;腺體:t值分彆為8.285、7.098,6.798、7.308,8.574、7.933,6.798、7.308,P值均＜0.05);伴鼻息肉CRS與不伴鼻息肉CRS組間差異無統計學意義(上皮:t值分彆為0.734、0.415、0.572、0.248,P值均＞0.05;腺體:t值分彆為0.331、0.662、0.249、0.644,P值均＞0.05).HIF-1α蛋白在伴或不伴鼻息肉CRS組織中較正常鼻竇黏膜顯著增高(t值分彆為3.522、3.314,P值均＜0.05).HIF-1α、MUC5AC、MUC5B mRNA在伴或不伴鼻息肉CRS患者中相對錶達量分彆為1.35±0.84、1.36±0.94,0.81±0.54、0.78±0.46,1.13±1.00、1.22±1.02,在健康對照組的相對錶達量為0.43±0.34、0.42 ±0.36、0.48±0.42,二者相比差異有統計學意義(t值分彆為4.087、4.089,2.519、2.550,2.738、2.955,P值均＜0.05).伴鼻息肉CRS患者HIF-1α與MUC5AC、MUC5B mRNA呈正相關(r值分彆為0.474、0.464,P值均＜0.05);不伴鼻息肉的CRS患者HIF-1α與MUC5AC、MUC5B mRNA呈正相關(r值分彆為0.477、0.514,P值均＜0.05).結論 HIF-1α、MUC5AC和MUC5B在伴或不伴鼻息肉的CRS患者中錶達升高,提示CRS是高黏液性疾病,且HIF-1 0與其中高分泌性的黏蛋白MUC5AC、MUC5B存在密切的關繫.
목적 연구결양유도인자1α(hypoxia inducible factor-1α,HIF-1 α)재만성비-비두염(chronic rhinosinusitis,CRS)중적표체급여점단백MUC5AC、MUC5B적관계.방법 선취23례반비식육적CRS환자、20례불반비식육적CRS환자화15례무CRS적정상비두점막,분별채용과전산-설부염색(periodic acid-schiff stain,PAS)검측각조중총점단백적표체,면역조직화학법(immunohistochemistry,IHC)、반정량반전록취합매련반응법(RT-PCR)분별검측HIF-1 α급점단백MUC5AC、MUC5B적표체,면역인기법(Western Blot법)검측각조중HIF-1α표체정황.이SPSS 16.0연건통계분석HIF-1 α여각점단백mRNA표체적상관성.결과 HIF-1α、MUC5AC、MUC5B、총점단백주요표체우반혹불반비식육CRS환자적점막상피급선체내,차교건강대조조명현증다,차이유통계학의의(상피:t치분별위8.650、9.305,9.382、8.524,7.533、5.417,5.507、5.556,P치균＜0.05;선체:t치분별위8.285、7.098,6.798、7.308,8.574、7.933,6.798、7.308,P치균＜0.05);반비식육CRS여불반비식육CRS조간차이무통계학의의(상피:t치분별위0.734、0.415、0.572、0.248,P치균＞0.05;선체:t치분별위0.331、0.662、0.249、0.644,P치균＞0.05).HIF-1α단백재반혹불반비식육CRS조직중교정상비두점막현저증고(t치분별위3.522、3.314,P치균＜0.05).HIF-1α、MUC5AC、MUC5B mRNA재반혹불반비식육CRS환자중상대표체량분별위1.35±0.84、1.36±0.94,0.81±0.54、0.78±0.46,1.13±1.00、1.22±1.02,재건강대조조적상대표체량위0.43±0.34、0.42 ±0.36、0.48±0.42,이자상비차이유통계학의의(t치분별위4.087、4.089,2.519、2.550,2.738、2.955,P치균＜0.05).반비식육CRS환자HIF-1α여MUC5AC、MUC5B mRNA정정상관(r치분별위0.474、0.464,P치균＜0.05);불반비식육적CRS환자HIF-1α여MUC5AC、MUC5B mRNA정정상관(r치분별위0.477、0.514,P치균＜0.05).결론 HIF-1α、MUC5AC화MUC5B재반혹불반비식육적CRS환자중표체승고,제시CRS시고점액성질병,차HIF-1 0여기중고분비성적점단백MUC5AC、MUC5B존재밀절적관계.
Objective To investigate the expression of hypoxia inducible factor-1α (HIF-1α) in chronic rhinosinusitis (CRS) and relationship with mucin MUC5AC,MUC5B secretion.Methods The expression of HIF-1 α,MUC5 AC and MUC5 B were measured by immunohistochemistry (IHC),Western Blot and reverse transcription polymerase chain reaction (RT-PCR) in CRS with or without nasal polyps (CRSwNP:twenty-three or CRSsNP:twenty)and fifteen normal sinus mucosa without CRS.The relationship between HIF-1α and MUC5AC,MUC5B were analyzed by SPSS 16.0 software.Results PAS and IHC showed that HIF-1α,MUC5AC,MUC5B,total mucins were expressed in nuclear of epithelium and gland cells,which were higher than normal control (t values in epithelium cells were 8.650,9.305,9.382,8.524,7.533,5.417,5.507,5.556,all P ＜ 0.05 ; t values in gland cells were 8.285,7.098,6.798,7.308,8.574,7.933,6.798,7.308,all P ＜ 0.05),but there was no significant difference between CRSwNP and CRSsNP (t values in epithelium cells were 0.734,0.415,0.572,0.248,all P ＞ 0.05 ; t values in gland cells were 0.331,0.662,0.249,0.644,P ＞ 0.05).Western Blot revealed that HIF-lα protein in CRSwNP and CRSsNP were higher than in normal contral (t values were 3.522,3.314,respectively,P ＜ 0.05).The relative expression levels of HIF-1α,MUC5AC,MUC5B mRNA in CRSwNP and CRSsNP were 1.35 ± 0.84,1.36 ± 0.94,0.81 ± 0.54,0.78 ± 0.46,1.13 ± 1.00,1.22 ± 1.02.But the relative expression levels of HIF-1 α,MUC5AC,MUC5 B mRNA in control subjects were 0.43 ± 0.34,0.42 ± 0.36,0.48 ± 0.42.There were significant differences between the groups of CRSwNP and control subiects(t values were 4.087,4.089,2.519,2.550,2.738,2.955,respectively,all P ＜ 0.05).There was a positive correlation among the expression of HIF-1 α and MUC5AC,5 B mRNA in CRSwNP (r values were 0.474,0.464,respectively,all P ＜0.05).There was a positive correlation among the expression of HIF-1α and MUC5AC,5B mRNA in CRSsNP (r values were 0.477,0.514,respectively,all P ＜ 0.05).Conclusions HIF-1 α,MUC5AC and MUC5B expression were upregulated in CRS with or without nasal polyps,indicating CRS is a disease of mucus hypersecretion and there is a close association between HIF-1α with hypersecretion of MUC5AC and MUC5B in mucus.
