CAJ | 학술논문

目的：采用高效液相色谱法测定不同来源不同采收期忍冬藤和金银花中绿原酸的含量。方法：色谱柱：Waters C18（250 mm ×4.6 mm，5μm）；流动相：乙腈-0.4%磷酸溶液（10：90）；检测波长：327 nm；流速：1.0 ml·min-1；柱温：30℃。以外标法测定6个不同采收期、3种来源的忍冬藤和金银花中绿原酸的含量。结果：绿原酸进样量在0.065~1.300μg范围内与峰面积呈良好的线性关系（ r=0.9998）。9、10月采收的忍冬藤中绿原酸的含量最高，三种来源的忍冬藤中无毛淡红忍冬藤中绿原酸的含量最高。结论：忍冬藤中绿原酸的含量高低与采收时间有一定的关系，本研究为忍冬藤药材的采收加工提供了一定的理论依据。
목적：채용고효액상색보법측정불동래원불동채수기인동등화금은화중록원산적함량。방법：색보주：Waters C18（250 mm ×4.6 mm，5μm）；류동상：을정-0.4%린산용액（10：90）；검측파장：327 nm；류속：1.0 ml·min-1；주온：30℃。이외표법측정6개불동채수기、3충래원적인동등화금은화중록원산적함량。결과：록원산진양량재0.065~1.300μg범위내여봉면적정량호적선성관계（ r=0.9998）。9、10월채수적인동등중록원산적함량최고，삼충래원적인동등중무모담홍인동등중록원산적함량최고。결론：인동등중록원산적함량고저여채수시간유일정적관계，본연구위인동등약재적채수가공제공료일정적이론의거。
Objective:To establish an HPLC method for the determination of chlorogenic acid in honeysuckle stem and honeysuck-le from different sources in different harvest periods. Methods:A Waters C18 column(250 mm × 4. 6 mm,5 μm)was used. The mo-bile phase consisted of acetonitrile-0. 4% H3 PO4(10:90)with the flow rate of 1. 0 ml·min-1 . The detection wavelength was 327 nm and the column temperature was 30℃. An external standard method was established for the determination of chlorogenic acid in honey-suckle stem and honeysuckle in six different harvest periods from three sources. Results:There was a good linear relationship within the range of 0. 065-1. 300 μg for chlorogenic acid(r=0. 999 8). The content of chlorogenic acid in honeysuckle was the highest in Sep-tember and October. The content of chlorogenic acid in Lonicera acuminata was the highest among the honeysuckle stem from three dif-ferent sources. Conclusion:The content of chlorogenic acid in honeysuckle has a certain relationship with the harvest time,which can provide theoretical basis for the choice of harvest time for honeysuckle stem.