中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
4期
679-681
,共3页
赵宏大%谢文%范文平%肖新月%孟淑芳
趙宏大%謝文%範文平%肖新月%孟淑芳
조굉대%사문%범문평%초신월%맹숙방
支原体检查%中国药典%药品检验
支原體檢查%中國藥典%藥品檢驗
지원체검사%중국약전%약품검험
Mycoplasma test%Chinese Pharmacopoeia%Drug testing
目的：为《中国药典》支原体检查法的进一步修订提供依据，为规范药品支原体检查试验操作提供参考意见。方法：比较有氧培养条件（aerobic conditions）与微有氧培养条件（microaerophilic conditions）下，口腔支原体（M. orale）和肺支原体（ M. pneumoniae）在液体和固体培养基上的生长差异。结果：两种支原体在有氧环境和微有氧环境的生长状况存在明显差距，支原体在微有氧环境的生长状态明显好于在有氧环境下。结论：支原体检查培养法应使用微有氧培养环境。《中国药典》支原体检查试验中对培养条件的规定不够明确，建议修订时应加以明确和规范。
目的：為《中國藥典》支原體檢查法的進一步脩訂提供依據，為規範藥品支原體檢查試驗操作提供參攷意見。方法：比較有氧培養條件（aerobic conditions）與微有氧培養條件（microaerophilic conditions）下，口腔支原體（M. orale）和肺支原體（ M. pneumoniae）在液體和固體培養基上的生長差異。結果：兩種支原體在有氧環境和微有氧環境的生長狀況存在明顯差距，支原體在微有氧環境的生長狀態明顯好于在有氧環境下。結論：支原體檢查培養法應使用微有氧培養環境。《中國藥典》支原體檢查試驗中對培養條件的規定不夠明確，建議脩訂時應加以明確和規範。
목적：위《중국약전》지원체검사법적진일보수정제공의거，위규범약품지원체검사시험조작제공삼고의견。방법：비교유양배양조건（aerobic conditions）여미유양배양조건（microaerophilic conditions）하，구강지원체（M. orale）화폐지원체（ M. pneumoniae）재액체화고체배양기상적생장차이。결과：량충지원체재유양배경화미유양배경적생장상황존재명현차거，지원체재미유양배경적생장상태명현호우재유양배경하。결론：지원체검사배양법응사용미유양배양배경。《중국약전》지원체검사시험중대배양조건적규정불구명학，건의수정시응가이명학화규범。
Objective:To provide the basic data for the further revision of mycoplasma test method described in Chinese Pharma-copoeia and some references for the operation standardization of drug mycoplasma test. Methods:Two incubation conditions,namely aerobic conditions and microaerophilic conditions,were compared with respect to the growth status of mycoplasma in liquid and solid media. Results:The growth of mycoplasma was obvious difference between the two incubation conditions,and the microaerophilic con-ditions were better than the aerobic conditions. Conclusion:The microaerophilic conditions can be used in the incubation of mycoplas-ma test,which should be defined and standardized in the future Chinese Pharmacopoeia.
