海洋学报（中文版）
海洋學報（中文版）
해양학보（중문판）
ACTA OCEANOLOGICA SINICA
2015年
4期
52-61
,共10页
长心卡帕藻%组织培养%愈伤组织%氧电极%光合与呼吸
長心卡帕藻%組織培養%愈傷組織%氧電極%光閤與呼吸
장심잡파조%조직배양%유상조직%양전겁%광합여호흡
Kappaphycus alvarezii%tissue culture%callus%oxygen electrode%photosynthesis and respiration
以 PES 液体培养基、固体培养基，采用正交设计，开展了长心卡帕藻（Kappaphycus alvarezii ）棕色藻株组织培养和愈伤组织诱导实验，探索了蔗糖、光强、植物生长调节剂对新芽形成和愈伤组织形成的影响，结果表明，固体培养基、IBA、6-BA 处理可诱导该藻产生愈伤组织，同时固体培养基更利于诱导形成愈伤组织；显微跟踪观察显示，该藻愈伤组织与高等植物的疏松愈伤组织不同，由细丝状细胞组成，系藻枝段中心髓部细胞脱分化形成的致密型愈伤组织；另外，愈伤枝段比新芽枝段有更高的光合和呼吸速率。
以 PES 液體培養基、固體培養基，採用正交設計，開展瞭長心卡帕藻（Kappaphycus alvarezii ）棕色藻株組織培養和愈傷組織誘導實驗，探索瞭蔗糖、光彊、植物生長調節劑對新芽形成和愈傷組織形成的影響，結果錶明，固體培養基、IBA、6-BA 處理可誘導該藻產生愈傷組織，同時固體培養基更利于誘導形成愈傷組織；顯微跟蹤觀察顯示，該藻愈傷組織與高等植物的疏鬆愈傷組織不同，由細絲狀細胞組成，繫藻枝段中心髓部細胞脫分化形成的緻密型愈傷組織；另外，愈傷枝段比新芽枝段有更高的光閤和呼吸速率。
이 PES 액체배양기、고체배양기，채용정교설계，개전료장심잡파조（Kappaphycus alvarezii ）종색조주조직배양화유상조직유도실험，탐색료자당、광강、식물생장조절제대신아형성화유상조직형성적영향，결과표명，고체배양기、IBA、6-BA 처리가유도해조산생유상조직，동시고체배양기경리우유도형성유상조직；현미근종관찰현시，해조유상조직여고등식물적소송유상조직불동，유세사상세포조성，계조지단중심수부세포탈분화형성적치밀형유상조직；령외，유상지단비신아지단유경고적광합화호흡속솔。
The brown morphotype of red seaweed Kappaphycus alvarezii collected from Li’an Bay in Lingshui County,Hainan Province of China,was used as explants in order to generate new buds and induce callus.Effect of the PES (Provasoli enriched seawater)media (with or without gelling agent),light intensity,and phytoregulators (Indole-3-acetic acid (IAA),Indole-3-butyric acid (IBA),6-benzylaminopurine (6-BA))were tested on new bud generation and callus formation by orthogonality experiment.The callus forming was observed in microscope,and the changes of the respiration rate and photosynthesis rate in explants among callus forming were detected.The callus of Kappaphycus alvarezii was achieved successfully by the combination of PES solid medium (0.8% ager), IBA,6-BA,while callus was not observed in the combination of PES liqiud medium,IBA and 6-BA.That indicates the solid medium plays an important role in callus induction.Kappaphycus alvarezii callus which consists of com-pact filamentous cells was different from the loose callus of superior plants.And callus derived from the medullary cells.It was found that both photosynthetic rate and respiratory rate in the branch inducing callus were higher than ones of the branch germinating new bud by means of oxygen electrode.
