CAJ | 학술논문

目的：建立同时测定扑麻滴鼻液中盐酸麻黄碱和马来酸氯苯那敏含量的HPLC方法。方法：采用：Purospher STAR RP18色谱柱（150 mm ×4.6 mm，5μm），以磷酸盐缓冲液（ pH 3.0±0.1）-乙腈（82：18）为流动相，流速为1.0 ml·min-1，在210 nm波长处同时检测盐酸麻黄碱和马来酸氯苯那敏，柱温35℃，进样量20μl。结果：盐酸麻黄碱和马来酸氯苯那敏分别在19.81~118.85μg·ml-1和6.21~37.25μg·ml-1浓度范围内线性关系良好，r分别为0.9996和0.9998。平均回收率分别为100.39%（RSD=0.69%，n=9）和100.11%（RSD=0.60%，n=9）。结论：该方法快速简便、重复性、准确性好，可用于该制剂中盐酸麻黄碱和马来酸氯苯那敏的含量测定。
목적：건립동시측정복마적비액중염산마황감화마래산록분나민함량적HPLC방법。방법：채용：Purospher STAR RP18색보주（150 mm ×4.6 mm，5μm），이린산염완충액（ pH 3.0±0.1）-을정（82：18）위류동상，류속위1.0 ml·min-1，재210 nm파장처동시검측염산마황감화마래산록분나민，주온35℃，진양량20μl。결과：염산마황감화마래산록분나민분별재19.81~118.85μg·ml-1화6.21~37.25μg·ml-1농도범위내선성관계량호，r분별위0.9996화0.9998。평균회수솔분별위100.39%（RSD=0.69%，n=9）화100.11%（RSD=0.60%，n=9）。결론：해방법쾌속간편、중복성、준학성호，가용우해제제중염산마황감화마래산록분나민적함량측정。
Objective:To establish a method for the simultaneous determination of chlorphenamine maleate and ephedrine hydro-chloride in chlorphenamine maleate and ephedrine nasal drops by HPLC. Methods:An HPLC method was performed on a column of Purospher STAR RP18(150 mm × 4. 6 mm,5 μm)with the mobile phase of acetonitrile -potassium dihydrogen phosphate buffer(pH 3. 0 ± 0. 1)(18:82 v/v)at the detection wavelength of 210 nm(adjusting the flow rate and column temperature to 1. 0 ml·min-1 and 35℃,respectively). The injection volume was 20 μl. Results:A good linear relationship was established between the peak response and the concentration of ephedrine hydrochloride and chlorphenamine maleate over the range of 19. 81-118. 85μg·ml-1(r=0. 999 6) and 6. 21-37. 25 μg·ml-1(r=0. 999 8),respectively. The mean recovery of ephedrine hydrochloride and chlorphenamine maleate was 100. 39%(RSD=0. 69%,n=9)and 100. 11%(RSD=0. 60%,n=9),respectively. Conclusion:The proposed method shows high repeatability,good durability and promising accuracy. It can be employed for the determination of two components in chlorphena-mine maleate and ephedrine nasal drops.