CAJ | 학술논문

目的：建立卡波姆类口腔黏附片中盐酸利多卡因的HPLC含量测定方法。方法：通过氯化钙沉淀供试品中的卡波姆，采用Welch C18色谱柱（250 mm ×4.6 mm，5μm），流动相为醋酸钠溶液（取冰醋酸50 ml，加水930 ml，用1 mol·l-1氢氧化钠调pH至3.40）-乙腈（70：30）；流速1.0 ml·min-1，检测波长254 nm，柱温40℃，进样量20μl。结果：在0.10~2.00 mg· ml-1的范围内，盐酸利多卡因的浓度与峰面积呈良好的线性关系（r=0.9999），平均回收率为100.2%，RSD为1.4%（n=9）。结论：本方法简单、准确、重复性好，可用于口腔黏附片中盐酸利多卡因的含量测定。
목적：건립잡파모류구강점부편중염산리다잡인적HPLC함량측정방법。방법：통과록화개침정공시품중적잡파모，채용Welch C18색보주（250 mm ×4.6 mm，5μm），류동상위작산납용액（취빙작산50 ml，가수930 ml，용1 mol·l-1경양화납조pH지3.40）-을정（70：30）；류속1.0 ml·min-1，검측파장254 nm，주온40℃，진양량20μl。결과：재0.10~2.00 mg· ml-1적범위내，염산리다잡인적농도여봉면적정량호적선성관계（r=0.9999），평균회수솔위100.2%，RSD위1.4%（n=9）。결론：본방법간단、준학、중복성호，가용우구강점부편중염산리다잡인적함량측정。
Objective:To establish a method to determine the content of lidocaine hydrochloride in buccal adhesive tablets contai-ning carbomer as an excipient by HPLC. Methods:Calcium chloride was used to precipitate carbomer in buccal adhesive tablets. The HPLC analysis was performed on a Welch C18 column(250 mm × 4. 6 mm,5 μm)with the column temperature of 40℃. The mobile phase was the mixture of 0. 05% sodium acetate solution(added 50 ml acetic acid into 930 ml distilled water,adjusting pH to 3. 40 with 1 ml·min-1 sodium hydroxide solution)and acetonitrile(70:30). The flow rate was 1. 0 ml·min-1 ,the detection wavelength was at 254 nm and the injection volume was 20 μl. Results:Within the range of 0. 10-2. 00 mg·ml-1 ,there was a good linear rela-tionship between the concentration and the peak area of lidocaine hydrochloride(r=0. 999 9). The average recovery was 100. 2%( RSD=1. 4%,n=9). Conclusion:The established method is simple,accurate and reproducible,which can be applied to determine the content of lidocaine hydrochloride in buccal adhesive tablets.