CAJ | 학술논문

对香榧主产地浙江省诸暨、绍兴、嵊州、东阳、磐安的5个香榧古树群体共149个样品进行了SRAP标记分析，共检测到260个位点，其中多态位点170个，多态位点占为65.38%（PPL%）；群体观察到的等位基因数（Na）为1.6538，有效等位基因数（Ne）为1.2713，基因多样性指数（H）为0.1696，Shannon信息指数（I）为0.2664；群体总的基因多样性指数（Ht）平均为0.1694，分化系数（Gst）为0.1442，群体内基因多样度（Hs）为0.1450，群体间基因流（Nm）为2.9675；利用加权平均数法（UPGMA）对香榧古树居群间进行了聚类分析，结果东阳、嵊州两个居群先聚在一起，再与磐安居群聚在一起，而绍兴和诸暨居群聚在一起，最后再与磐安、东阳、嵊州居群聚在一起。研究结果表明，香榧古树接穗来源非唯一，是一个栽培类型，同时群体间有基因交流，既有分离又保持相对较高的相似性。
대향비주산지절강성제기、소흥、승주、동양、반안적5개향비고수군체공149개양품진행료SRAP표기분석，공검측도260개위점，기중다태위점170개，다태위점점위65.38%（PPL%）；군체관찰도적등위기인수（Na）위1.6538，유효등위기인수（Ne）위1.2713，기인다양성지수（H）위0.1696，Shannon신식지수（I）위0.2664；군체총적기인다양성지수（Ht）평균위0.1694，분화계수（Gst）위0.1442，군체내기인다양도（Hs）위0.1450，군체간기인류（Nm）위2.9675；이용가권평균수법（UPGMA）대향비고수거군간진행료취류분석，결과동양、승주량개거군선취재일기，재여반안거군취재일기，이소흥화제기거군취재일기，최후재여반안、동양、승주거군취재일기。연구결과표명，향비고수접수래원비유일，시일개재배류형，동시군체간유기인교류，기유분리우보지상대교고적상사성。
Leaves from 149 ancient Torreya grandis cv. Merrillii trees were collected in 5 counties (cities) such as Dongyang (DY), Pan’an (PA), Shengzhou (SZ), Zhuji (ZJ) and Shaoxing (SX), Zhejiang province. SRAP (sequence related amplified polymorphism) analysis on 149 samples resulted that a total of 260 loci was detected, among them 170 of polymorphic (Np), accounting for 65.38%of the total one. Number of alleles (Na) was 1.653 8, number of effective alleles (Ne) 1.271 3, Nei’s gene diversity index (H) 0.1696, Shannon Information index (I) 0.266 4, total gene diversity (Ht) 0.1694, genetic differentiation (Gst) 0.144 2, gene diversity within population (Hs) 0.145 0 and gene flow (Nm) 2.967 5. Cluster analysis indicated that the scions of the old trees may not be from the same source and there were gene exchanges among populations, which led to separation but relatively high identity.